Important notice
The course guide is provisional.
The PDF version of the course guide may take a few days to become available in the DDD.

Integrated Laboratory for Reproduction Biology
Code: 42949Credits: 9
| Degree programme | Type | Course |
|---|---|---|
| Cytogenetics and Reproductive Biology | OP | 1 |
Contact lecturer
- Name :
- Carme Nogues Sanmiquel
- Email :
- carme.nogues@uab.cat
Teaching staff
- Ignasi Roig Navarro
- Zaida Sarrate Navas
- Elena Ibañez De Sans
- Andreu Blanquer Jerez
Group languages
You can consult this information at the end of the document.
Prerequisites
This subject has no prerequisites.
Objectives
The module "Integrated Laboratory of Reproductive Biology" aims to give basic tools to students in order to acquire the ability to develop the tasks carried out in Assisted Reproductive Centers and in research laboratories focused in cell culture and reproduction.
In the submodule 1: "Embryonic Stem Cell (ESC) culture" students will acquire the skills necessary to work in a cell culture laboratory. They will learn the rules and they will get used to work in sterile conditions. They will also learn the basic techniques of protein detection and acquire the ability to use standard and inverted microscope and fluorescence microscope. They will learn to differentiate between pluripotent and differentiated ESC.
In the submodule 2: “Fluorescent in vitro hybridization in spermatozoa” students will learn how to analyze the chromosomal abnormalities in sperm from a sample of semen, through the technique of FISH and to make a clinical evaluation.
In the submodule 3: "Oocyte and Embryo culture" students will acquire the skills to work in a laboratory of reproductive biology. They will learn how to obtain and manipulate oocytes and embryos, activate oocytes and isolate blastomeres.
In the submodule 4: "Update in histological and cytological techniques," students will learn the basic techniques of histology such as inclusion, microtomy, staining, and protein detection and to observe the samples obtained.
Finally, in the submodule 5: "Confocal Laser Scanning Microscopy" (CLSM) they will learn the characteristics of this microscope, as well as the advantages and limitations and how to use it.
Learning outcomes
- CA16 (Plan the management of research laboratories and clinical laboratories in the area of reproductive biology, taking into account quality and safety criteria.) Plan the management of research laboratories and clinical laboratories in the area of reproductive biology, taking into account quality and safety criteria.
- CA17 (Design scientific experiments in the field of the reproductive biology laboratory, selecting appropriate methodologies and tools to respond to research hypotheses.) Design scientific experiments in the field of the reproductive biology laboratory, selecting appropriate methodologies and tools to respond to research hypotheses.
- KA21 (Relate the cellular and molecular foundations of reproductive biology with experimental procedures applied in laboratory mammals.) Relate the cellular and molecular foundations of reproductive biology with experimental procedures applied in laboratory mammals.
- SA22 (Apply cell culture and characterisation techniques in the reproductive biology laboratory.) Apply cell culture and characterisation techniques in the reproductive biology laboratory.
- SA23 (Apply the principles of quality, biosafety and traceability when carrying out activities, using resources and monitoring work flows in an integrated reproductive biology laboratory.) Apply the principles of quality, biosafety and traceability when carrying out activities, using resources and monitoring work flows in an integrated reproductive biology laboratory.
- SA24 (Analyse research data in an integrated reproductive biology laboratory.) Analyse research data in an integrated reproductive biology laboratory.
- SA25 (Demonstrate communication skills, both oral and written, to communicate the results obtained in a clinical or research reproductive biology laboratory.) Demonstrate communication skills, both oral and written, to communicate the results obtained in a clinical or research reproductive biology laboratory.
Contents
Submodule 1: embryonic stem cells (ESC) cultures
- STO (feeders) cultures.
- STO inactivation.
- Coculture ESC/STO.
- Detection of pluripotency (immunofluorescence).
- ESC differentiation.
- Detection of differentiation (immunofluorescence)
- Capture and analysis of images of the different cell types and of the immunofluorescence
Submodule 2: Fluorescent in situ hybridization on sperm
- Fluorescent in situ hybridization technique in a fixed semen sample
- Evaluation of hybridization quality
- Analysis of chromosomal abnormalities in the sample
Submodule 3: Mouse oocytes and embryos culture
- Mouse embryos collection and culture.
- Embryo partition
- Mouse oocytes collection and in vitro maturation.
- Mouse oocyte activation
Submodule 4: Update on histological and cytological techniques
- Development of histological technique: inclusion and microtomy.
- Staining of histological samples of ovary and/or testicle.
- Flow cytometry and its use in research.
- Microscopic visualization and digital imaging.
- Processing images using Photoshop / Image J - FIJI.
Submodule 5: Confocal laser scanning microscopy
- Basics of Fluorescence and Confocal Microscopy
- Sample preparation for fluorescence
- Capturing the image in Confocal Microscope
- Processing series
Learning activities and methodology
| Title | Hours | ECTS | Learning outcomes |
|---|---|---|---|
| Confocal Laser Scanning Microscopy | 10 | 0.4 | CA16, SA22, SA23 |
| Update in histological and cytological techniques | 20 | 0.8 | CA16, SA22, SA23 |
| How to prepare a Photographic composition | 8 | 0.32 | CA17, SA24, SA25 |
| Oocyte and Embryo culture | 10 | 0.4 | CA16, SA22, SA23 |
| Personalized tutorials | 30 | 1.2 | KA21, SA24 |
| Sperm fluorescent in situ hybridization | 5 | 0.2 | CA16, SA22, SA23 |
| How to prepare a laboratory report | 10 | 0.4 | SA24, SA25 |
| How to solve problems and case studies | 10 | 0.4 | CA17, SA24, SA25 |
| Solve problems and case studies | 8 | 0.32 | CA17, KA21, SA25 |
| Embryonic Stem Cell (ESC) culture | 15 | 0.6 | CA16, SA22, SA23 |
| Study | 73 | 2.92 | CA16, CA17, KA21, SA22, SA23, SA24 |
| Photographic composition using Photoshop software | 8 | 0.32 | CA17, KA21, SA25 |
| Laboratory reports | 8 | 0.32 | CA17, KA21, SA25 |
This course is essentially practical. In all submodules except confocal laser scanning microscopy students will work in pairs under the guidance of a teacher.
In the embryonic stem cells culture submodule, students must acquire the ability to work in sterile conditions.
In the FISH submodule, students will learn to process semen samples to apply FISH methodologies and to identify chromosomal abnormalities.
In the mouse embryo culture submodeule, the practical classes are designed to acquire the skills necessary to handle oocytes and embryos.
In the histology submodeule students will become familiar with the techniques used in histology.
Finally, in the scanning laser confocal microscopy submodeule, students must work in groups of approximately 6 people. This practice is carried out in Microscopy Service, using the laser scanning confocal microscopes available in the service.
Assessment
Continuous assessment activities
| Title | Weight | Hours | ECTS | Learning outcomes |
|---|---|---|---|---|
| Delivery reports | 67% | 2 | 0.08 | CA17, KA21, SA24 |
| Acquiring skills in stem cell culture laboratory | 6% | 1 | 0.04 | CA16, CA17, SA22, SA23, SA24, SA25 |
| Individual tests | 8% | 4 | 0.16 | CA16, CA17, KA21, SA24, SA25 |
| Solve problems and case studies | 10% | 1 | 0.04 | CA16, CA17, KA21, SA23, SA24 |
| Acquiring skills in using a confocal microscope | 2% | 1 | 0.04 | CA17, KA21, SA22, SA23, SA24, SA25 |
| Acquiring skills in histology techniques | 7% | 1 | 0.04 | CA17, KA21, SA23, SA24, SA25 |
This module consists of five submodules, each with a different dedication and therefore a specific percentage within the module. In the following table you will find a summary of the hours of each submodule and its weight in the final mark of the module:
| hours | % | ||
| 1 | ESC cultures | 15 | 25 |
| 2 | FISH-in spermatozoa | 5 | 8 |
| 3 | Oocytes and embryos culture | 10 | 17 |
| 4 | Update in histological and cytological techniques | 20 | 33 |
| 5 | Confocal laser scanning microscopy | 10 | 17 |
| 60 | 100 |
Evaluation activities scheduled:
Submodule 1. Embryonic stem cells culture. This submodule has a weight of 25% of the module. The evaluation system is organized into two sections: 1) attitude and skills acquired in the laboratory (15%) and 2) resolution of a case study (85%)
Submodule 2. Spermatozoa fluorescent in situ hybridization. This submodule has a weight of 8% of the module. The evaluation will consider the final report explaining the results (100%)
Submodule 3. Oocytes and embryos culture. This submodule has a weight of 17% of the module. The evaluation will consider the final laboratory report (100%)
Submodule 4. Update on histological and cytological techniques. This submodule has a weight of 33% of the module. The evaluation system is organized into three sections: 1) skills acquired in practical sessions (20%), 2) delivery of an individual report and questionnaires (40%) and 3) delivery of a photographic composition using the program Photoshop (40%)
Submodule 5. Confocal laser scanning microscopy. This submodule has a weight of 17% of the module. The evaluation system is organized into three sections: 1) skills acquired in practical sessions (10%) and 2) resolution of a case study 90%)
The final grade will be calculated taking into account the percentage of the different submodules. To pass the module the student must obtain a minimum mark of 5 points and a maximum of 10 possible points. For the different submodules tobe averaged, a minimum grade ≥4 must be obtained in each of the submodules. Scores lower than ≤3,99 in one or more of the submodules will require students to pass a retake an exam of all submodules.
To be eligible for the retake process, the student should have been previously evaluated in a set of activities equaling at least two thirds of the final score of the course or module. Thus, the student will be graded as \"No Avaluable\" if the weighthin of all conducted evaluation activities is less than 67% of the final score.
The faculty responsible for each of the submodules in this practical module will inform the students about how they may use artificial intelligence.
Bibliography
- Barnes, David W. & Mather, Jennie P. (1998). Animal cell culture methods.Academic Press
Disponible en paper a la biblioteca
- Barnes, David W. [i altres]. (1998). Animal cell culture methods. Academic Press Barn
Disponible en línia
- Behringer, Richard. (2014). Manipulating the mouse embryo : a laboratory manual. (4th ed.) Cold Spring Harbor Laboratory Press
Disponible en paper a la biblioteca
- Freshney, R. Ian. (2010). Culture of animal cells : a manual of basic technique and specialized applications. (6th ed.) Wiley-Blackwell
Disponible en paper a la biblioteca
- Freshney, R. Ian. (2016). Culture of animal cells : a manual of basic technique and specialized applications. (7th ed.) Wiley-Blackwell
Disponible en línia
- Griffiths, J. B. & Doyle, A. (1998). Cell and tissue culture : laboratory procedures in biotechnology. John Wiley & Sons
Disponible en paper a la biblioteca
- Suvarna, S. Kim & Layton, Christopher & Bancroft, John D. (2013). Bancroft's theory and practice of histological techniques. (7th ed.) Churchill Livingstone Elsevier
Disponible en línia
Software
Free software of image analysis Image J - FIJI.
Imaris Viewer (versió lliure del Imaris; Oxford Instruments)
Course groups and languages
The information provided is provisional until November 30. After this date, you will be able to consult the language of each group through this link. To access the information, you will need to enter the course CODE
| Type of teaching | Group | Language | Semester | Shift |
|---|---|---|---|---|
| (PLABm) Practical laboratories (master) | 1 | Catalan/Spanish | second semester | morning-mixed |
| (SEMm) Seminars (master) | 1 | Catalan | second semester | morning-mixed |