Important notice
The course guide is provisional.
The PDF version of the course guide may take a few days to become available in the DDD.
Industrial Production of Bioproducts
Code: 45555Credits: 9
| Degree programme | Type | Course |
|---|---|---|
| Advanced Biotechnology | OP | 1 |
Contact lecturer
- Name :
- Oscar Romero Ormazabal
- Email :
- oscar.romero.ormazabal@uab.cat
Teaching staff
- Xavier Garcia Ortega
- Francesc Gòdia Casablancas
- Laura Cervera Gracia
Group languages
You can consult this information at the end of the document.
Prerequisites
To attend the module it is necessary to have a basic formation in Biochemical Engineering, in fundamental aspects of Bioprocess Engineering, in Bioreactors and some basic concepts of recombinant DNA and Genetic Engineering.
Objectives
The objective of this module is to familiarize the student with the most important tools used in a bioprocess, and its application in the design and operation of bioprocesses in their future professional careers. In order to achieve this objective, different cellular factories will be explored, designed, integrated and optimized for producing industrial biotechnological products, integrating the production and purification of the bioproduct in a reproducible way (BIOPAT concept) and economically viable Bioprocess Engineering. The quality and safety regulations of bioproducts from different fields will also be explained and the principles on which the scale up of a bioprocess is based will be presented.
Learning outcomes
- CA10 (Integrate and justify the use of different Biotechnology and Bioprocess Engineering tools to solve emerging problems in industrial biotechnology.) Integrate and justify the use of different Biotechnology and Bioprocess Engineering tools to solve emerging problems in industrial biotechnology.
- CA11 (Integrate knowledge and address the complexity of formulating judgments based on information that, despite being incomplete or limited, includes reflections on the social and ethical responsibilities associated to the application of such knowledge and judgments.) Integrate knowledge and address the complexity of formulating judgments based on information that, despite being incomplete or limited, includes reflections on the social and ethical responsibilities associated to the application of such knowledge and judgments.
- CA12 (Integrate and synthesize information obtained from the scientific literature using appropriate channels, contrasting alternatives and engaging in critical debate on the matter.) Integrate and synthesize information obtained from the scientific literature using appropriate channels, contrasting alternatives and engaging in critical debate on the matter.
- KA12 (Contrast the advantages, disadvantages and bioprocess engineering required in the prokaryotic E. coli cell factory, the eukaryotic P. pastoris cell factory and the animal cell factory.) Contrast the advantages, disadvantages and bioprocess engineering required in the prokaryotic E. coli cell factory, the eukaryotic P. pastoris cell factory and the animal cell factory.
- SA10 (Search, compare, critically analyse, and synthesize information obtained from databases and other sources to solve complex problems in one’s field.) Search, compare, critically analyse, and synthesize information obtained from databases and other sources to solve complex problems in one’s field.
- SA11 (Prepare technical reports in the fields of environmental and/or biological engineering and communicate the results orally in a clear, concise and unambiguous manner.) Prepare technical reports in the fields of environmental and/or biological engineering and communicate the results orally in a clear, concise and unambiguous manner.
- SA12 (Plan different task resolution activities entrusted within a working group, while adequately managing time and resources.) Plan different task resolution activities entrusted within a working group, while adequately managing time and resources.
- SA13 (Assess scale-up challenges in Biotechnology.) Assess scale-up challenges in Biotechnology.
Contents
1.- Introduction to the industrial production of bioproducts. Scale up in bioreactors
2.- Bioprocess design based on quality.
2.1.- Quality by Design (QbD) / Process Analytical Technology (PAT)
2.2.-Good Manufacturing Practice (GMPs). Good Laboratory Practices (BPLs),
3.-Cell factories: Animal cell culture
4.-Cell factories: Pichia pastoris.
5.-Cell factories: Escherichia coli.
6.- Case of study workshop
Learning activities and methodology
| Title | Hours | ECTS | Learning outcomes |
|---|---|---|---|
| Workshop report | 15 | 0.6 | CA10, CA12, SA11 |
| Case study workshop | 20 | 0.8 | CA10, CA11, CA12, KA12, SA11, SA13 |
| Case study preparation | 10 | 0.4 | CA10, CA11, KA12, SA10, SA12 |
| Lectures | 33.5 | 1.34 | CA10, CA11, KA12, SA12, SA13 |
| writing work and oral exposure | 24.5 | 0.98 | CA10, CA12, SA10, SA12 |
| seminars | 4 | 0.16 | CA10, CA11, SA12, SA13 |
| group work | 35 | 1.4 | CA10, CA11, CA12, KA12, SA10, SA12 |
| Study | 50 | 2 | CA11, KA12, SA12 |
| Search of documentation and bibliography | 28 | 1.12 | CA10, CA11, CA12, SA10, SA13 |
Lectures on the topics of the syllabus.
Seminars on aspects of the industrial world of Biotechnology by experts invited from the sector.
Elaboration of gruop works. Group activity. Students will prepare a report on a topic related to the contents, at the teacher's proposal. These works will be exposed and defended in public.
Case study workshop. Students will carry out workshops on the different cell factories, consisting offamiliarization with a recombinant protein production process, cell cultures and bioprocess monitoring. In addition, a visit will be made to a biotechnology company related to the course theme.
The use of AI is permitted to improve the drafting and style of the reports. However, generating original content with AI is strictly prohibited. If AI tools are used, their use must be explicitly declared, and it must be clearly identified within the report what they were used for.
Assessment
Continuous assessment activities
| Title | Weight | Hours | ECTS | Learning outcomes |
|---|---|---|---|---|
| 4 Writing exams | 13,1% each | 3 | 0.12 | CA10, CA11, CA12, KA12, SA13 |
| Assesment of worshop | 25 | 1 | 0.04 | CA12, KA12, SA11, SA12, SA13 |
| Assessment of oral presentation | 22,5% | 1 | 0.04 | CA10, CA11, CA12, KA12, SA10 |
Evaluation of the theoretical part of the module:
Continuous assessment
Individual written evaluation: It is 70% of the final grade. Four partial tests corresponding to different subjects of the course are carried out with a weight of 25% each of them. If in the individual written evaluation the student obtained a grade lower than 3/10, he will not pass the module.
Evaluation of the defense and oral presentation of a research paper (30%)
Final evaluation:
Students who do not pass the continuous assessment will have a global test of written individual final recovery. Whenever this test is exceeded with a grade higher than 3/10, it will be done with the grade of the oral presentation.
Global evaluation of the module
Evaluation of workshop (25%). Minimum note of this part to approve trhe module 3.5/10
Evaluation of the theoretical part of the module (75%). Minimum note of this part to approve trhe module 3.5/10
The calendar of exams and the different activities to be carried out in the module will be announced at the beginning of the course. Once scheduled, in no case will exams be held with different dates and times.
For the review of the results of the evaluations, the time and manner will be fixed within 10 working days after the communication of the results through the virtual platform. If the student does not show up for this review, this activity will not be subsequently reviewed.
Matriculation of Honor (MH). Awarding the grade of MH is the decision of the faculty responsible for the subject. UAB regulations state that MHs can only be awarded to students who obtain a final grade equal to or higher than 9.00. Up to 5% of the total number of students may be awarded MHs.
A student is considered as not evaluable (NA) if he/she has not shown up for any of the evaluation activities.
Regardless of other disciplinary measures that may be considered appropriate, irregularities committed by students that may result in a modification of the grade of an evaluation activity will be graded with a zero. Therefore, copying, plagiarism, cheating, allowing copying, etc... in any of the evaluation activities will imply a zero grade.
Bibliography
López Santín, Josep et al. Ingeniería bioquímica / Eds: Francesc Gòdia Casablancas, Josep López Santín. Madrid: Síntesis, 2010.
Bailey, James E. (James Edwin), and David F Ollis. Biochemical Engineering Fundamentals / James E. Bailey, David F. Ollis. 2nd ed. New York [etc: McGraw-Hill, 1986.
Doran, Pauline M. Bioprocess Engineering Principles. 2nd ed. San Diego: Elsevier Science & Technology, 2012.
Villadsen, John, ed. Fundamental Bioengineering / Ed. John Villadsen. 1st ed. Weinheim, Germany: Wiley-VCH, 2016.
Additional bibliography, mainly scientific articles, necessary for following the module can be consulted through the virtual platform. In parallel, the student will have to carry out specific bibliographic searches and consultations for the preparation of their group work.
Software
It is not planned to use any specific software for the subject.
Course groups and languages
The information provided is provisional until November 30. After this date, you will be able to consult the language of each group through this link. To access the information, you will need to enter the course CODE
| Type of teaching | Group | Language | Semester | Shift |
|---|---|---|---|---|
| (TEm) Theory (master) | 1 | Catalan | first semester | afternoon |
| (PLABm) Practical laboratories (master) | 1 | Catalan/Spanish | first semester | morning-mixed |
| (SEMm) Seminars (master) | 1 | Catalan | first semester | afternoon |