Important notice
The course guide is provisional.
The PDF version of the course guide may take a few days to become available in the DDD.

Applied Biocatalysis and Biotransformations
Code: 42901Credits: 9
| Degree programme | Type | Course |
|---|---|---|
| Advanced Biotechnology | OP | 1 |
Contact lecturer
- Name :
- Oscar Romero Ormazabal
- Email :
- oscar.romero.ormazabal@uab.cat
Teaching staff
- Marina Guillen Montalban
- Oscar Romero Ormazabal
Group languages
You can consult this information at the end of the document.
Prerequisites
Basic knowledge of:
- Protein structure
- Biochemistry
- Catalysis
- Kinetic enzyme
- Microbial growth
- Cell biology
- Chemical Engineering principles
Objectives
The objective is to present biocatalysis and its fields of application as an alternative to classical catalysis. The knowledge of biocatalysts will be deepened as the central axis of a transformation and of the aspects of engineering of the main elements that make up biotransformation, such as the biocatalyst, the reaction medium, the bioreactor and its operation. Moreover, the classification and study of different biotransformations according to the biocatalyst used will be analyzed in different case studies. The final objective is to be able to define the main elements of a biotransformation and to design a biocatalytic process.
Learning outcomes
- CA06 (Implement a biocatalytic process for industrial application, integrating the current state of biocatalysis, its fields of application and the different classes of relevant biotransformations.) Implement a biocatalytic process for industrial application, integrating the current state of biocatalysis, its fields of application and the different classes of relevant biotransformations.
- CA07 (Explain the different types of biotransformation depending on the biocatalyst used, evaluating their main characteristics.) Explain the different types of biotransformation depending on the biocatalyst used, evaluating their main characteristics.
- KA04 (Gather relevant information to determine the most appropriate operational configuration for a biotechnological process.) Gather relevant information to determine the most appropriate operational configuration for a biotechnological process.
- KA05 (Differentiate the fundamental concepts of chemical engineering in the different forms of reactor design and operation, including catalytic reactors, with special emphasis on reactors with immobilized biological catalysts.) Differentiate the fundamental concepts of chemical engineering in the different forms of reactor design and operation, including catalytic reactors, with special emphasis on reactors with immobilized biological catalysts.
- KA06 (Distinguish and choose between different reaction media used in biotransformations.) Distinguish and choose between different reaction media used in biotransformations.
- KA07 (Characterise biocatalyst immobilisation and distinguish between its different methods.) Characterise biocatalyst immobilisation and distinguish between its different methods.
- KA08 (Identify biomolecules and multi-enzyme processes of relevance to biotechnological applications, considering their function in biological systems and their possible role in human pathologies.) Identify biomolecules and multi-enzyme processes of relevance to biotechnological applications, considering their function in biological systems and their possible role in human pathologies.
- SA06 (Calculate the main elements involved in biotransformation such as the biocatalyst, reaction medium and bioreactor, as well as their operation.) Calculate the main elements involved in biotransformation such as the biocatalyst, reaction medium and bioreactor, as well as their operation.
Contents
Assignment program:
- Topic 1 Introduction to biocatalysis
- Topic 2 Biocatalysts
- Topic 3 Biocatalyst engineering
- Topic 4 Reaction medium engineering
- Topic 5 Bioreactor operation
- Topic 6 Biotransformations: case studies
Learning activities and methodology
| Title | Hours | ECTS | Learning outcomes |
|---|---|---|---|
| Individual study | 127 | 5.08 | CA06, CA07, KA04, KA05, KA06, KA07, KA08, SA06 |
| theoretical classes | 49 | 1.96 | KA04, KA05, KA06, KA07, KA08 |
| Accomplishment of work on a case study | 20 | 0.8 | CA06, KA04, KA05, KA06, KA07, KA08, SA06 |
| Seminars and case studies | 6 | 0.24 | CA06, CA07, KA04, KA05, KA06, KA07, KA08, SA06 |
| Search for documentation and bibliography | 20 | 0.8 | CA06, CA07, KA04, KA05, KA06, KA07, KA08 |
Directed activities:
- Theoretical lectures: Master classes on the concepts of the subject
- Seminars: Presentation to students of different cases of study of biotransformations and applied biocatalysis extracted from the bibliography
- Public presentation of the work: Students will orally and publicly present a summary of the most relevant results of the work and deliver the presentation to the professor in digital format through the virtual campus
Autonomous activities:
- Individual study: Individual study, preparation of diagrams and summaries.
- Documentation and bibliography search: Consultation of the bibliographic and documentary sources essential for the course.
- Realization of a work: work in groups of 2-4 students in which each group will elaborate a written work on a case study previously selected by the teacher. The work will be delivered to the teacher in paper format (printed) and in digital format through the virtual campus, in order to discuss in seminars and written presentation.
Communication with Students
Communication with students will take place through the Moodle Classroom on the Virtual Campus.
Informative note:
The use of AI is permitted to improve the drafting and style of the reports. However, generating original content with AI is strictly prohibited. If AI tools are used, their use must be explicitly declared, and it must be clearly identified what they were used for.
Assessment
Continuous assessment activities
| Title | Weight | Hours | ECTS | Learning outcomes |
|---|---|---|---|---|
| Written work | 20% | 0 | 0 | CA06, CA07, KA04, KA05, KA06, KA07, KA08, SA06 |
| Oral and public presentation of the work | 10% | 1 | 0.04 | CA06, CA07, KA04, KA05, KA06, KA07, KA08, SA06 |
| Partial exam (topics 4, 5 and 6) | 35% | 1 | 0.04 | CA07, KA05, KA06, KA07, KA08, SA06 |
| Partial exam (items 1, 2 and 3) | 35% | 1 | 0.04 | KA05, KA07, KA08 |
Scheduled evaluation process and activities
Throughout the course different evaluation activities will be carried out that will lead to the final grade of the subject obtained by continuous evaluation. Specifically, the evaluable activities will be:
- Written work on a case study that is 20% of the final grade.
- Oral and public presentation of the written work that is 10% of the final grade
- Attendance at all oral presentations is compulsory for all students
- Partial exam of topics 1, 2 and 3, which represents 35% of the final grade
- Partial examination of topics 3, 4 and 5, which represents 35% of the final grade
The written assignment and the oral presentation of the assignment are non-recoverable.
The course is considered passed if the average of the 4 activities is 5 or higher. To calculate the average, each activity must have a minimum grade of 4.
If the average of the 4 activities is less than 5, or any part is below 4, the failed activities (those with less than 5) must be retaken, except the written assignment and the oral presentation, which are non-recoverable.
Any of the following circumstances will result in a “Not Assessable” grade for the course:
- Not submitting the written assignment
- Not doing the oral presentation
- Not attending any of the exams
Scheduling of assessment activities
At the beginning of the course, groups will be formed for the written assignment. The deadlines for submission and oral presentations will be communicated through the virtual campus.
Recovery process
The student may attend the recovery session only if they have completed a set of activities representing at least two-thirds of the total course grade.
Partial exams are eliminatory; therefore, a student who has passed a partial exam cannot retake it. They must retake any partial exam in which they obtained a grade below 4, regardless of their overall average, as outlined in the “Scheduled Evaluation Process and Activities” section.
The final grade will be calculated in the same way as for continuous assessment.
Grade review procedure
For each assessment activity, a time, date, and location will be set for the student to review their work with the teacher. During this review, students may raise complaints about their grade, which will be considered by the course instructors. If the student does not attend the review session, the activity will not be reviewed afterward.
Grading
The decision to award an “Honors” distinction is up to the course instructors. UAB regulations state that an Honors grade can only be awarded to students with a final grade equal to or higher than 9.00. A maximum of 5% of the enrolled students may receive this distinction.
Student misconduct, cheating, and plagiarism
Without prejudice to other disciplinary measures deemed appropriate, any irregularities committed by a student that could lead to a variation in the grade of an assessment activity will result in a grade of zero. Therefore, cheating, plagiarism, deception, or allowing others to copy in any evaluation activity will result in a zero and the activity will be non-recoverable.
If the course requires passing one of these affected activities, the entire course will be failed directly, without the opportunity for recovery during the same academic year. In this situation, the final grade recorded will be 2.
Evaluation of repeating students
There is no different evaluation system for repeating students.
Single assessment
This course does not have a single-assessment option.
Bibliography
Books:
Immobilized Biocatalysts
Peter Grunwald (Ed.)
Pages: 510
Published: November 2018
ISBN 978-3-03897-318-8 (Pbk); ISBN 978-3-03897-319-5 (PDF)
https://doi.org/10.3390/books978-3-03897-319-5
© 2018 by the authors; CC BY-NC-ND licenes
https://www.mdpi.com/books/pdfview/book/861
Biocatalysis for Green Chemistry and Chemical Process Development
Editor(s): Junhua (Alex) Tao, Romas Kazlauskas
First published:12 April 2011
Print ISBN:9780470437780 |Online ISBN:9781118028308 |DOI:10.1002/9781118028308
Copyright © 2011 John Wiley & Sons, Inc.
https://onlinelibrary-wiley-com.are.uab.cat/doi/book/10.1002/9781118028308
Carrier‐bound Immobilized Enzymes: Principles, Application and Design
Author(s): Dr. Linqiu Cao
First published:29 September 2005
Print ISBN:9783527312320 |Online ISBN:9783527607662 |DOI:10.1002/3527607668
Copyright © 2005 Wiley‐VCH Verlag GmbH & Co. KGaA
https://bibcercador.uab.cat/permalink/34CSUC_UAB/cugbhl/alma991010341558406709
Green Biocatalysis
Editor(s): Ramesh N. Patel
First published:13 May 2016
Print ISBN:9781118822296 |Online ISBN:9781118828083 |DOI:10.1002/9781118828083
Copyright © 2016 John Wiley & Sons, Inc.
https://cataleg.uab.cat/iii/encore/record/C__Rb2087415?lang=cat
Autor | |
Títol | Enzyme structure and mechanism / Alan Fersht |
Edició | 2nd. ed. |
Publicació/producció | New York : W.H. Freeman, cop. 1985 |
Descripció | xxi, 475 p.; 24 cm |
Matèria | |
ISBN | 0716716143 |
0716716151 (pbk.) |
https://bibcercador.uab.cat/permalink/34CSUC_UAB/cugbhl/alma991002478739706709
Autor | |
Títol | Enzymes / by Malcolm Dixon and Edwin C. Webb |
Edició | 3rd ed. |
Publicació/producció | London : Longman, 1979 |
Descripció | XXIII + 1116 p.; 24 cm |
Matèria |
https://bibcercador.uab.cat/permalink/34CSUC_UAB/cugbhl/alma991000758989706709
Títol | Enzyme biocatalysis : principles and applications / Andrés Illanes, editor |
Publicació/producció | [Dordrecht] : Springer, 2008 |
Descripció | X, 391 p. : ill. ; 25 cm. |
Matèria | |
ISBN | 9781402083600 |
https://bibcercador.uab.cat/permalink/34CSUC_UAB/cugbhl/alma991008142159706709
Títol | Ingeniería bioquímica/Francesc Gòdia Casablancas y Josep López Santín (editores) ; Carles Casas Alvero ... [et al.] |
Publicació/producció | Madrid : Síntesis, DL 1998 |
Descripció | 350 p. : il.; 24 cm |
Col·lecció | Ciencias químicas (Síntesis). Tecnología bioquímica y de los alimentos |
Matèria | |
ISBN | 8477386110 |
http://cataleg.uab.cat/record=b1425826~S1*cat
Autor | |
Títol | Biocatalysis : [fundamentals and applications] / A.S.Bommarius, B.R.Riebel |
Publicació/producció | Weinheim : Wiley-VCH, 2004 |
Descripció | XXIII, 611 p.; 24 cm |
Matèria | |
ISBN | 3527303448 |
https://bibcercador.uab.cat/permalink/34CSUC_UAB/cugbhl/alma991010349685006709
Títol | Multi-step enzyme catalysis : biotransformations and chemoenzymatic synthesis / edited by Eduardo Garcia-Junceda |
Publicació/producció | Weinheim : Wiley-VCH ; Chichester : John Wiley, 2008 |
Descripció | 241 p. ; 25 cm |
Matèria | |
ISBN | 9783527319213 |
http://cataleg.uab.cat/record=b1747444~S1*cat
Títol | Immobilization of enzymes and cells / edited by Gordon F. Bickerstaff |
Publicació/producció | Totowa : Humana Press, 1997 |
Descripció | XIV, 367 p.;23 cm |
Col·lecció | |
Matèria | |
ISBN | 0896033864 |
https://bibcercador.uab.cat/permalink/34CSUC_UAB/cugbhl/alma991010401253806709
Autor | |
Títol | Biotransformations in organic chemistry : a textbook : with 37 figures, 238 schemes and 16 tables / Kurt Faber |
Edició | 5th revised and corrected ed. |
Publicació/producció | Berlin: Springer-Verlag, cop. 2004 |
Descripció | XI, 453 p. : il; 24 cm |
Matèria | |
ISBN | 3540200975 |
http://cataleg.uab.cat/record=b1615815~S1*cat
Autor | |
Títol | Biocatalysis : biochemical fundamentals and applications / Peter Grunwald |
Publicació/producció | London : Imperial College Press, 2009 |
Descripció | xvi, 1035 p. : il. ; 24 cm |
Matèria | |
ISBN | 9781860947711 |
1860947719 |
https://cataleg.uab.cat/record=b1778458~S1*cat
Kourist, R. (2015), Biocatalysis in Organic Synthesis. Science of Synthesis, Vol. 1–3. Edited by Kurt Faber, Wolf-Dieter Fessner and Nicholas J. Turner.. Angew. Chem. Int. Ed., 54: 12547. doi:10.1002/anie.201508130
Search engines of scientific bibliography:
Scholar Google: http://scholar.google.es/advanced_scholar_search?hl=en&lr=
Scopus: http://www.scopus.com/scopus/search/form.url?display=authorLookup
Scifinder: Software disponible a la UAB
Sience Direct: http://www.sciencedirect.com/science/journals
ISI Web of Knowledge: http://www.accesowok.fecyt.es/login/
Interesting web sites:
Base de dades d’enzims BRENDA: http://www.brenda-enzymes.info/
National Center for Biotechnology Information: http://www.ncbi.nlm.nih.gov/
ExPASy (Expert Protein Analysis System) Proteomic Server: http://www.expasy.ch/
Software
No software
Course groups and languages
The information provided is provisional until November 30. After this date, you will be able to consult the language of each group through this link. To access the information, you will need to enter the course CODE
| Type of teaching | Group | Language | Semester | Shift |
|---|---|---|---|---|
| (TEm) Theory (master) | 1 | Catalan | first semester | afternoon |
| (SEMm) Seminars (master) | 1 | Catalan | first semester | afternoon |