Logo

Integrated Laboratory

Code: 102612
Credits: 3
2026/2027
Degree programme Type Course
Veterinary Medicine OB 1

Contact lecturer

Name :
Anna Genesca Garrigosa
Email :
anna.genesca@uab.cat

Teaching staff

Maria Jose Docampo Garcia
Joaquín Ariño Carmona
Josep Maria Folch Albareda
Néstor Gomez Trias
Marcelo Amills Eras
Ferran Llobet Cabau
Diego Vargas Donayre
Sandra Andreu Cortés
Asier Gonzalez Sevine
Anna Genesca Garrigosa
Joan Repulles Fernandez
Maïmouna Asma Selmane

Group languages

You can consult this information at the end of the document.

Prerequisites

The student must be attending simultaneously or have taken the Bioquímica (102662) and Biologia Animal i Cel·lular (102652) subjects.

It is essential to review and assimilate the documentation available in the spaces of the Virtual Campus regarding safety in laboratories. In addition, practice laboratories can not be accessed without justification of having passed the basic laboratory safety test, available in the Virtual Campus.

The use of the gown and safety goggles will be mandatory, as well as following the regulations regarding the safety elements described in the practice guides.

The use of a dressing gown and, in some practical lessons, the use of safety glasses will be mandatory, as well as following the regulations regarding the safety elements described in the practice guides.

Objectives

The objectives of this course focus on the acquisition of competencies within the framework of students’ practical training: their introduction to laboratory work and their familiarization with the techniques most commonly used in the fields of Biochemistry, Molecular Biology, and Cell Biology.

Regarding the Biochemistry part, in these practices, some of the concepts explained in the theory classes are applied and expanded. Students will acquire the basic skills to work safely and autonomously in a laboratory, while acquiring the knowledge and skills necessary to perform dilution calculations accurately. Students will also consolidate the knowledge learned about the methods used in the manipulation and purification of proteins and nucleic acids. Likewise, through the determination of certain biological parameters and enzymatic kinetic constants, students will acquire knowledge about different analytical and spectroscopic methods that will be a reference in their future development as a professional in the field of veterinary medicine.

The practical component of Molecular Genetics applied to domestic species aims primarily to familiarize students with diagnostic techniques based on the use of molecular markers. Using pig blood samples and goat/cow milk samples, students must extract genomic DNA. Subsequently, the diagnosis of Porcine Stress Syndrome is performed using PCR-RFLP, and the origin of the milk sample (goat or cow) is determined using a species-specific PCR assay based on mitochondrial DNA analysis. Understanding and mastering these techniques is important both for the diagnosis of hereditary diseases and for certifying the origin of certain foods or products of pharmaceutical interest.

Regarding Cell Biology, the laboratory sessions focus on learning basic techniques specific to this field and on acquiring the skills and practices required for laboratory work. Specifically, the objectives are: to consolidate proficiency in the use of the light microscope and in sample preparation for this type of microscopy; to observe different types of cells and cellular structures; to learn how to interpret images obtained using different types of microscopes; to observe mitotic division in different cell types to understand the function of the mitotic spindle and the contractile ring; to observe meiotic division in germ cells and understand the processes of meiotic recombination; to understand how fluorescence microscopy works and its application to disease diagnosis; and finally, to observe the process of fertilization and the early stages of embryonic development.

Learning outcomes

  1. Analyse, synthesise and resolve problems and make decisions.
  2. Apply theoretical and practical experience of different basic biochemical procedures in the study of biological molecules.
  3. Demonstrate theoretical and practical experience of different biochemical procedures of concern as a support for diagnosis.
  4. Use theoretical knowledge and practical experience of different basic methodologies in the study of cells and their functions.
  5. Interpret and explain the functions of cells and basic cellular processes by means of practical experience.

Contents

The subject is structured in three blocks: 1) Biochemistry and Molecular Biology, 2) Molecular Genetics and 3) Cell Biology.


Biochemistry and Molecular Biology (24h)


first semester sessions (8 h + 9h)

BQ0: Description of the set of general safety standards in Biochemistry and Biology teaching laboratories.

BQ1: Separation of a mixture of amino acids by ion exchange chromatography (Dowex) and identification by thin-layer chromatography.

BQ2: Solutions and dilutions: Dilution techniques, calculation of concentrations and preparation of solutions.

BQ3: Solving problems in the calculation of solutions and dilutions.

BQ4: Study of renal function in dogs: determination of the protein/creatinine ratio in urine.

BQ5: Protein analysis with polyacrylamide gel electrophoresis and SDS

BQ6: Protein determination with the biuret method

BQ7: Determination of the Km of serum alkaline phosphatase.


Second-semester sessions

Sessions BQ8-BQ10 (LI6 in the timetable). Lab: V0-147 and computer room. (3.0h + 2.5h + 1.5h = 7.0h)

BQ8: Transformation of bacteria with plasmid DNA.

BQ9: Plasmidic DNA purification from bacteria (Miniprep) and DNA analysis by restriction.

BQ10: Bioinformatics applications.


Molecular Genetics (9 h)


GM1-5 (LI8 in the timetable). Lab: V0-207.

Molecular Genetics applied to domestic species (2h + 2h + 1.5h + 1.5h + 2h = 9h)


Cell Biology. Lab V0-120 (18h)


BC1. Optical microscopy

BC2. Electron microscopy. The contents of this session will be implemented in a practical classroom format.

BC3. Mitotic cell division

BC4. Fluorescence and confocal microscopy (3h)

BC5. Meiotic cell division (3h)

BC6. Fertilization and embryo development (3h).


Learning activities and methodology

Title Hours ECTS Learning outcomes
Laboratory practical sessions 51 2.04 1, 2, 3, 4, 5
Study 24 0.96 1, 2, 3, 4, 5

This is a practical subject in which no master lessons are given. Students perform the experimental work individually and under the supervision of the responsible teacher. However, part of the contents will be taught in seminar format.

The practice Guides, where the protocols of each practice are detailed and the questionnaires of response are presented, will be available in the Virtual Campus of the subject.

Before beginning each practical session the student must have read the protocol and know the objectives of the practice, the fundamentals, and the procedures that must be carried out.

The aim of the practical classes is to complete and reinforce the knowledge acquired in the theoretical classes and seminars of the subjects of Biochemistry and Animal and Cell Biology as well as to acquire and reinforce basic notions of Molecular Genetics. The practical sessions will stimulate the acquisition of skills such as the ability to observe as well as the analysis and interpretation of the results obtained.

At the beginning of each practice session, an introduction will be made explaining the theoretical foundations of the practice to be performed, the operation of the different devices that will be used during the practice, as well as the general safety regulations to be taken into account during its development.

In this subject, the use of Artificial Intelligence (AI) technologies is allowed as an integral part of the development of the work, provided that the final result reflects a significant contribution of the student in the analysis and personal reflection. The student must clearly identify which parts have been generated with this technology, specify the tools used and include a critical reflection on how these have influenced the process and the final result of the activity. The lack of transparency in the use of AI will be considered a lack of academic honesty and may lead to a penalty in the grade of the activity, or greater sanctions in serious cases.

Annotation: within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.

Assessment

Continuous assessment activities

Title Weight Hours ECTS Learning outcomes
Short tests at the end of each practice or block of practices. It is included within the described time of the practice session (individual assessment) Weighted average of the puntuation of the different tests, as described in "Evaluation" 0 0 1, 2, 3, 4, 5

The evaluation system for the acquisition of competencies for this subject is based on continuous assessment, based on written tests of different types, including specific questionnaires and the resolution of problems/cases related to the practices.

Given the practical nature of the subject, attendance at the sessions is mandatory. Unjustified absences may be tolerated by up to 10%. In any case, if the total number of absences (including justified and not justified) exceeds 20% of the total number of sessions, the student will be considered Not Evaluable.

The final score of the subject will be obtained exclusively from the weighted average, obtained by the continuous evaluation throughout the course, of the different contents. In order to pass the subject, it is necessary to obtain a final score of 5 or greater, provided that a minimum score of 3 has been obtained in each of the three parts of the subject. The contents of Biochemistry and Molecular Biology will contribute 47%, those of Molecular Genetics 18%, and those of Cell Biology 35% to the final score. It may be encouraged, if the teacher deems it appropriate, previous knowledge of the content of the practical sessions, through the procedures that the teachers determine. This factor can represent up to 10% of the score of that practice session.

Due to the experimental nature of this course, there will be no make-up or second-chance examinations.


Cell Biology

The practices will be evaluated through a multiple-choice test of approximately 15 minutes, which will be done in the laboratory at the end of each practice. The six exams will have the same weight and altogether will contribute 35% of the final grade of the subject.


Biochemistry and Molecular Genetics

Students will be assessed by means of short tests, either as a multiple-choice test and/or, in certain sessions, solving problems that require a short answer: two in the first term, practices LI1 (BQ1-BQ3) and LI2 (BQ4- BQ7), and two in the second semester, LI6 (BQ8-BQ10), LI8 (GM1-5). The weighting of the marks of the practices of Biochemistry and Molecular Genetics in the final mark will be as follows: the LI1 will contribute 16% of the final mark of the subject, the LI2 17%, the LI6 14% and the LI8 the remaining 18%. The performance of the tests will be mandatory, and the unperformed tests will be qualified with “zero”.

This subject does not offer the single assessment option.

Bibliography

Basic bibliography


* Nelson, D.L., y Cox, M.M. Lehninger Principios de bioquímica. 7ª edición. Ed Omega. 2018.
* Berg, J. M., Tymoczko, J. L. & Stryer, L. Bioquímica. 7ª edició. Ed. Reverté. Barcelona, 2013.
* Griffiths, AJ.F. Genética. 7ª edición. McGraw Hill/Interamericana de España ed. 2008.
* Voet, D., Voet, J.G i Pratt, C.W. Fundamentos de Bioquímica. 2ª edición. Ed. Panamericana. 2007.
* Kathi Canese and Sarah Weis. Chapter \"PubMed: The Bibliographic Database\" in the The NCBI Handbook. 2nd edition. Bethesda (MD): National Center for Biotechnology Information (US) http://www.ncbi.nlm.nih.gov/books/NBK153385/

Web links

Web pages for the DNA analysis and manipulation.

http://tools.neb.com/NEBcutter2/

http://molbiol-tools.ca/Restriction_endonuclease.htm

 

Database of biomedical literature

http://www.ncbi.nlm.nih.gov/pubmed/

 

The bibliography and the web links of the Cell Biology contents are indicated in the protocols of the practices or, where appropriate, in the Teaching Guide of the corresponding theoretical subject.

Software

No special software will be used

Course groups and languages

The information provided is provisional until November 30. After this date, you will be able to consult the language of each group through this link. To access the information, you will need to enter the course CODE

Type of teaching Group Language Semester Shift
(PLAB) Practical laboratories 1 Catalan/Spanish annual morning-mixed
(PLABs) Suport a les pràctiques de laboratori 1 Catalan/Spanish annual morning-mixed
(PLAB) Practical laboratories 2 Catalan/Spanish annual morning-mixed
(PLABs) Suport a les pràctiques de laboratori 2 Catalan/Spanish annual morning-mixed
(PLAB) Practical laboratories 3 Catalan/Spanish annual morning-mixed
(PLABs) Suport a les pràctiques de laboratori 3 Catalan/Spanish annual morning-mixed
(PLAB) Practical laboratories 4 Catalan/Spanish annual morning-mixed
(PLABs) Suport a les pràctiques de laboratori 4 Catalan/Spanish annual morning-mixed
(PLAB) Practical laboratories 5 Catalan/Spanish annual morning-mixed
(PLABs) Suport a les pràctiques de laboratori 5 Catalan/Spanish annual morning-mixed
(PLAB) Practical laboratories 6 Catalan/Spanish annual morning-mixed
(PLABs) Suport a les pràctiques de laboratori 6 Catalan/Spanish annual morning-mixed
(PLAB) Practical laboratories 7 Catalan/Spanish annual morning-mixed
(PLABs) Suport a les pràctiques de laboratori 7 Catalan/Spanish annual morning-mixed