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Integrated Laboratory III

Code: 101945
Credits: 3
2026/2027
Degree programme Type Course
Genetics OB 2

Contact lecturer

Name :
María Pilar Garcia Guerreiro
Email :
mariapilar.garcia.guerreiro@uab.cat

Teaching staff

Lidia Gonzalez Quereda
Jordi Corral Sabado

Group languages

You can consult this information at the end of the document.

Prerequisites

  • It is mandatory to have taken -o being currently taking- the theoretical subjects related to the experimental work developed here. 
  • Biosecurity and security tests at 'campus virtual' need to be passed. The student must prove knowledge and acceptance of the Bioscience laboratory guidelines.
  • It is necessary to go through the theoretical content of each module before the day of the practical classes.
  • Wearing a lab coat is mandatory. It is not possible to enter to the lab without a lab coat.
  • Attendance is mandatory.
  • Students should come to the class following the assigned schedule. Changes in the original schedule need to be approved by the corresponding professor and in all cases before the starting of the classes.

 

Objectives

The Integrated Laboratory III is the third course in a set of 6 which are distributed along 6 semesters of the first three courses of the degree of genetics. These subjects aim to give a solid foundation of experimental procedures, techniques and skills of genetics and related sciences. The practical work help to reinforce the theoretical concepts acquired in the theory, and allow us to understand the essential dialogue between theory and experimentation that have given rise to the body of knowledge that constitutes the science of genetics.

The Integrated Laboratory III has as objectives the acquisition of experimental skills in 4 specific modules of content:

  • Molecular Genetics of Eukaryotes
  • Molecular Biology of Prokaryotes
  • Cytogenetics
  • Scientific Documentation

Objectives Molecular Genetics of Eukaryotes:

  • Understand and use the basic nucleic acids’ extraction, manipulation and amplification techniques.
  • Understand how to use different detection techniques of DNA sequences.
  • Apply the acquired knowledge to design an experiment in the field of Genetics.
  • Learn how to decide the appropriate experimental techniques to develop a research project.

Objectives Molecular Biology of Prokaryotes:

The module of Molecular Biology of Prokaryotes gives the basics prokaryotes genetics. Their specific objectives are as follows:

  • Know how to use the various techniques of incorporation of exogenous DNA in prokaryotes.
  • Learn about systems that allow for the obtaining of new bacterial strains by mutagenesis processes in random or targeted mutagenesis.
  • Know how to use tools that allow the study of gene expression in prokaryotes.
  • Acquire skills in working with biosafety cabinets in a microbiology laboratory, applied to the study of bacterial pathogenicity using cell line models.

Objectives of Cytogenetics:

  • Learning of the protocols for chromosomal preparations.
  • Learning techniques for chromosome identification.
  • Check the effects of ionizing radiation on the karyotype.


Objectives of Scientific Documentation:

The general objective is to provide a theoretical and practical base of knowledge that will enable the student to acquire basic information skills related to the search and information retrieval, as well as the resolution of information needs intrinsic to the scientific activity.

Specific objectives:

  • Know the types of sources of bibliographic information.
  • Learn how to solve information needs in the field of genetics and related using the most appropriate bibliographical resources.
  • Know strategies to search and retrieve bibliographic information in electronic format.
  • Gain criteria to question the reliability of the bibliographical sources.


Learning outcomes

  • CM18 (Integrate experimental techniques and bioinformatics tools for the collection, processing and preliminary analysis of biological data) Integrate experimental techniques and bioinformatics tools for the collection, processing and preliminary analysis of biological data
  • CM19 (Design experimental strategies and data analysis procedures for solving research problems in genetics.) Design experimental strategies and data analysis procedures for solving research problems in genetics.
  • CM20 (Evaluate the relevance, limitations, and biological significance of data obtained using experimental and computational techniques.) Evaluate the relevance, limitations, and biological significance of data obtained using experimental and computational techniques.
  • KM13 (Identify the fundamentals of experimental techniques in molecular genetics, genomics and proteomics, as well as the use of databases and associated bioinformatics tools.) Identify the fundamentals of experimental techniques in molecular genetics, genomics and proteomics, as well as the use of databases and associated bioinformatics tools.
  • KM14 (Describe the methodologies of sample preparation, cytogenetics and microscopy applied to the diagnosis and analysis of human genetic variation.) Describe the methodologies of sample preparation, cytogenetics and microscopy applied to the diagnosis and analysis of human genetic variation.
  • SM16 (Apply safety, biosafety and waste management protocols in the handling and processing of biological samples.) Apply safety, biosafety and waste management protocols in the handling and processing of biological samples.
  • SM17 (Use bioinformatics tools and databases for the management, analysis and simulation of genetic and genomic data.) Use bioinformatics tools and databases for the management, analysis and simulation of genetic and genomic data.

Contents

Module Molecular Genetics of Eukaryotes


• Session 1: Genomic DNA extraction and amplification by PCR.


• Session 2: Agarose gel electrophoresis. DNA Dot Blot preparation and hybridization.


• Session 3: Plasmid DNA extraction and enzymatic digestion.


• Session 4: Dot blot hybridization and multiple-choice assessment test.




Module Molecular Biology of prokaryotes


• Session 1 (5 h) transfer of genetic material in prokaryotes.


• Session 2 (3 h). Processes of mutagenesis and recombination to obtain new strains.


• Session 3 (4 h). Use of mobile genetic elements to obtain mutants.


• Session 4 (3 h). Control of gene expression in prokaryotes.


• Session 5 (3h). Study of bacteria–host interactions using cell line.



Module cytogenetics


• First session


General introduction to practices


Preparation of chromosome spreads of human monocytes


• Second session


Uniform staining: microscopic analysis


Uniform staining: evaluation of the effects of ionizing radiation


• Third session


Human karyotype: features and nomenclature


G Bands: microscopic analysis


• Fourth session


Fluorescent in situ hybridization (FISH) on extensions of human chromosomes



Module Scientific Documentation


The module is structured in two sessions, of two hours each, which will be held in computerized classrooms.




Session 1. The bibliographic search. Retrieval of information in bibliographic catalogues: the search engine of the UAB Library Service.


Session 2. Bibliographic search in science and technology databases, repositories and academic search engines.

Learning activities and methodology

Title Hours ECTS Learning outcomes
Libreta Laboratori 2 0.08 KM14
Tutories individuals 2 0.08 CM18, CM20, KM13
Laboratori Mòdul Biologia Molecular de Procariotes 15 0.6 CM18, CM19, CM20, KM13, KM14, SM16
Laboratori Mòdul Citogenètica 15 0.6 CM18, CM19, CM20, KM13, KM14, SM16
Aula d'Informàtica Mòdul Documentació Científica 4 0.16 SM17
Laboratori Módul Genètica Molecular d'Eucariotes 15 0.6 CM18, CM19, CM20, KM13, KM14, SM16
Estudi 16 0.64 CM18, CM19, CM20, KM13, KM14, SM16

The subject is taught in small groups of students (maximum 20 per session) in the laboratory. Students have a manual or practice guide for each Module. It is necessary to read the corresponding part of each session carefully before starting the practice to obtain the maximum advantage. Students will have to produce the results obtained.


Molecular Genetics of Eukaryotes

Autonomous learning in which students learn through hands-on work, guided throughout by the supervising instructor, who sets the objectives for the practical sessions. Students will work with various molecular biology techniques to obtain and interpret results, ultimately answering multiple-choice questions designed to assess their understanding of the topics covered across the four sessions. Work will be carried out in groups of two or three, alternating between experimental benchwork and theoretical explanations regarding the techniques and concepts involved. Attendance at all four practical sessions is mandatory, except for duly justified reasons.


Molecular Biology of Prokaryotes

The module will be taught in the laboratory and in small groups of students (with a maximum of 22 students per session).

Students will have before the completion of the sessions associated to the Molecular Biology module of Procariotes of a specific Practices Manual that they will find in the Virtual Campus or where the teaching staff indicates.

To carry out the practices associated to the module of Molecular Biology of Procariotes the students will work in pairs.

At the beginning of each session the teacher will make a brief theoretical explanation of the content of the practices that will be carried out as well as the experiences to be carried out by the students.

In order to achieve good performance and acquire the competencies corresponding to this module, it is essential that the student read a comprehensive reading of the Practices Manual, familiarizing with the practices that will be carried out and with the methodology that will be applied in each case.


Cytogenetics Module


The Cytogenetics module will be given at the Faculty integrated teaching laboratories. In these sessions the students will be divided into three groups.

The student must consult which group they belong to and attend classes corresponding to the assigned group.

The students will work in pairs and will have to attend the group of practices assigned obligatorily.

Only occasional changes will be accepted as long as they are balanced (a student from a group for a student from another group).

If a student has not been able to perform a practice session with his group, he can retrieve it by attending another group, as long as the group in question has free spaces.


The students will have a guideline of practices (Virtual Campus of the subject) that they will have to carry out to carry out the practices.

To facilitate the understanding of the contents and a good development of the classes it is advisable for the student to read the practice guideline before each session.


During the elaboration of the practices the students will have to solve in person exercises facilitated by the professor.

These exercises must be delivered at the end of each session.


Module Scientific Documentation


Each session will include a brief part of theoretical exposition and a second of a practical type where the student will have to perform exercises related to the consultation of bibliographic sources and

the resolution of informational needs.

The issues to be resolved will always be presented contextualized within the thematic field of genetics related sciences.


Annotation: within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.

Assessment

Continuous assessment activities

Title Weight Hours ECTS Learning outcomes
Biología Molecular de Eucariotas. Qüestionaris. Avualuació continuada dels resultats experimentals 30% 1.8 0.072 CM18, CM19, CM20, KM13, KM14, SM16
Citogenètica. Qüestionaris. Avaluació continuada dels resultats experimentals 30% 1.8 0.072 CM18, CM19, CM20, KM13, KM14, SM16
Documentació Científica 10% 0.6 0.024 SM17
Biologia Molecular de Procariotes. Qüestionaris. Avaluació continuada dels resultats experimentals 30% 1.8 0.072 CM18, CM19, CM20, KM13, KM14, SM16

Molecular Genetics of Eukaryotes Evaluation

Attendance at the practical sessions is mandatory and, therefore, an absence without a justified reason may lead to the module not being evaluated. A justified reason is understood to be health problems (the corresponding medical certificate must be brought to the practical session coordinator) or serious personal problems. In this case, the practical session will be made up whenever possible. The practical sessions will be assessed through an exam that will be held at the end of the 4th session and will represent 80% of the final grade. The student's attitude and work in the laboratory will also be assessed, representing 20% ​​of the total grade. To pass the module, the questionnaire must be passed with a grade higher than 5.

Molecular Biology of Procaryotes Module

In the evaluation of the Molecular Biology module of Procaryotes, two aspects will be taken into account: on the one hand, the note obtained in a questionnaire that will be done at the end of session 5 and which will refer to all the practices that make up this module, and On the other, the achievement of the objectives set in each of the programmed practices will also be valued.

The questionnaire will represent 70% of the final grade of the module, while the remaining 30% will depend on the evaluation of the results obtained and the experimental work performed.

To pass the module, students must obtain a score higher than 5 on the questionnaire.

Cytogenetics Module

The module's note will be obtained through the arithmetic mean of the four exercises performed during the laboratory sessions. Taking into account the mandatory nature of the practices, the lack of unjustified assistance implies a penalty in the final note of the module:

- Missing one day implies a reduction of 30% in the practice note.

- Missing two days implies 60% reduction in the practice note.

- Missing to three or more days implies a zero in the practice note.

This penalty is exempt from those students who can not attend the session of their group for just cause. Health problems are justified because the medical certificate must be carried out by the practice coordinator or serious personal problems. In this case, the practice will recover the practice whenever possible.

Scientific Documentation Module

To pass the module it is essential to attend both sessions, deliver within the established period the two practical exercises provided and conduct a written test. The attendance and realization of the practical exercises supposes 20% of the final qualification. The written test means the remaining 80%.

To pass the subject, it is necessary to first approve each module with a mark ≥ 5. Students who do not pass the different modules of the subject or want to improve their note can present themselves on the scheduled date for the subject's recovery evaluation. The resentment of the student in the examination of improvement of note entails the renunciation of the qualification previously obtained. Students who have not passed one of the modules after the recovery assessment will not approve the subject. However, it will not be necessary for a repeat student to carry out the teaching activities or the evaluations of that module passed after the second enrolment. Repeaters will only have to evaluate the concrete module that they have not passed. This exemption will be maintained for a period of three additional license plates. The final grade is the average of the notes of each module.

Not evaluable

The \"Not Evaluable\" rating will be obtained when the number of evaluation activities performed is less than 50% of the programmed ones.

To be eligible for the retake process, the student should have been previously evaluated in a set of activities equalling at least two thirds of the final score of the course or module. Thus, the student will be graded as \"No Avaluable\" if the weigh thin of all conducted evaluation activities is less than 67% of the final score.

Attendance to practical sessions is mandatory. Students missing more than 20% of programmed sessions will be graded as \"No Avaluable\".

Students who take the single assessment must do the laboratory practices in face-to-face sessions, as they are teaching activities with compulsory attendance. This unique assessment system will apply to modules 1 (Molecular Biology of Eukaryotes), 2 (Molecular Biology of Prokaryotes) and 3 (Cytogenetics), and will consist of a single synthesis test with test-type questions or development on the contents of the practical activities. The grade obtained in this summary test will have the same weight in the final grade as those established for the continuous assessment.

The single assessment test will take place on a date agreed between the students, the subject coordinator and those responsible for each module.

The same criterion will be applied to approve the subject and the same recovery system as for the continuous assessment. The review of the final qualification follows the same procedure as for the continuous assessment.

Module 4 (Scientific Documentation) does not provide for the single assessment system.


Any irregularity committed in an assessment activity (academic fraud, plagiarism, or improper use of AI, unless such use is expressly authorized in the study guide) that may lead to a significant alteration of the grade will result in that assessment activity being graded with a 0. If the study guide establishes that obtaining a minimum grade in that assessment activity is an essential requirement for passing the course, or if multiple irregularities occur in the assessment activities of the same course, the final grade for the course will be 0. In addition, disciplinary proceedings may be initiated against any student who engages in any of these irregularities.

Bibliography

Molecular Genetics of Eukaryotes

Included in the laboratory workbook, which is available on the Virtual Campus.

Molecular Biology of Prokaryotes

Laboratory workbook available on the Virtual Campus


Cytogenetics

Laboratory workbook available on the Virtual campus.


Scientific Documentation

  1. ABADAL, E.; CODINA, Ll. Bases de datos documentales: características, funciones y método. Madrid: Síntesis, 2005.
  2. ALEIXANDRE, R. “Fuentes de información en ciencias de la salud en Internet” [En línia]. Panace@, 2011, vol. 11, núm. 33. [Consulta: 11-07-2014]. Disponible a: http://www.medtrad.org/panacea/IndiceGeneral/n33-Ponencias-Aleiandre.pdf
  3. CASTILLO, L. “Tema 3: fuentes y recursos de referencia” [En línia]. [Consulta: 09-07-2014]. Disponible a: http://www.uv.es/macas/SR3.pdf
  4. CORDÓN, J.A, et al. Nuevas fuentes de información: información y búsqueda documental en el contexto de la web 2.0. Madrid: Pirámide, 2012.
  5. Fuentes de información biomédica [En línia]. Cedimcat. [Consulta: 09-07-2014]. Disponible a: http://www.cedimcat.info/html/es/dir2471/doc26734.html
  6. GALLEGO, J.; JUNCA, M. “Fuentes de información en ciencias sociales y humanidades, ciencias de la saludy cienciay tecnología” [En línia]. Edukanda: recursos informativos en red. 2010, 17 de juny. [Consulta: 06-07-2014]. Disponible a: http://www.edukanda.es/mediatecaweb/data/swf/633.swf
  7. HERNANDEZ-PEREZ, T.; GARCIA-MORENO, M.A. \"Datos abiertos y repositorios de datos: nuevo reto para los bibliotecarios\" [En línia]. El profesional de la informacion, 2013, v. 22, n. 3. [Consulta: 09-07-2014]. Disponible a: http://eprints.ucm.es/22025/
  8. JUNCA, M. “Análisis de contenido: resumen e indización” [En línia]. Edukanda: recursos informativos en red. 2010, 16 de juny. [Consulta: 06-07-2014]. Disponible a: http://www.edukanda.es/mediatecaweb/data/swf/592.swf
  9. JUNCA, M. “Sistemas de clasificación documentales” [En línia]. Edukanda: recursos informativos en red. 2010, 16 de juny. [Consulta: 06-07-2014]. Disponible a: http://www.edukanda.es/mediatecaweb/data/swf/594.swf
  10. TORRES RAMIREZ, I. Las fuentes de información: estudios teórico-prácticos. Síntesis

Software

No specific software is needed.

Course groups and languages

The information provided is provisional until November 30. After this date, you will be able to consult the language of each group through this link. To access the information, you will need to enter the course CODE

Type of teaching Group Language Semester Shift
(PLAB) Practical laboratories 621 Catalan/Spanish first semester morning-mixed
(PLABs) Suport a les pràctiques de laboratori 621 Catalan/Spanish first semester morning-mixed
(PLAB) Practical laboratories 622 Catalan/Spanish first semester morning-mixed
(PLABs) Suport a les pràctiques de laboratori 622 Catalan/Spanish first semester morning-mixed
(PLAB) Practical laboratories 623 Catalan/Spanish first semester morning-mixed
(PLABs) Suport a les pràctiques de laboratori 623 Catalan/Spanish first semester morning-mixed