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Separation and Purification Processes

Code: 100959
Credits: 6
2026/2027
Degree programme Type Course
Biotechnology OB 3

Contact lecturer

Name :
Oscar Romero Ormazabal
Email :
oscar.romero.ormazabal@uab.cat

Teaching staff

Yerko Fredes Tapia
Sady Roberto Rodriguez Avila

Group languages

You can consult this information at the end of the document.

Prerequisites

It is recommended to have the foundations of bioprocess engineering achieved.

Objectives

The study of the different separation processes used in biotechnology, its theoretical basis, the dimensioning of the equipment and the development of the strategies and the sequences to be applied according to the product to obtain.

Learning outcomes

  • CM21 (Design an industrial process taking into account ethical and sustainable development aspects.) Design an industrial process taking into account ethical and sustainable development aspects.
  • KM19 (Define the units, variables and characteristics of the phenomena of matter and energy transport.) Define the units, variables and characteristics of the phenomena of matter and energy transport.
  • KM20 (Define the diversity of separation processes at various scales in bioprocess engineering.) Define the diversity of separation processes at various scales in bioprocess engineering.
  • SM19 (Use a bioreactor appropriately.) Use a bioreactor appropriately.

Contents

1. Introduction.

2. Filtration.

3. Centrifugation and sedimentation.

4. Cell disruption.

5. Liquid-liquid extraction.

6. Adsorption.

7. Chromatography.

8. Membrane separation processes.

9. Precipitation.

10. Crystallization.

11. Drying.

Learning activities and methodology

Title Hours ECTS Learning outcomes
Soving practical exercises and correction 18 0.72 CM21, KM19, KM20
Practical exercises 15 0.6 CM21, KM19, KM20
Resolution of practical exercises 21 0.84 CM21, KM19, KM20, SM19
Studi 33 1.32 CM21, KM19, KM20
Seminars 5 0.2 CM21, KM19, KM20
Tutories 4 0.16 CM21, KM19, KM20
homeworks 15 0.6 CM21, KM19, KM20
Theoretical lectures 30 1.2 CM21, KM19, KM20

Theoretical lectures:

The basic concepts of the syllabus will be introduced. Whenever possible, audiovisual and interactive material to help understand concepts will be used. This material will be available in Moodle before the theoretical sessions are held

Practical excercises:

The teacher and the students will solve problems related to the subject exposed in the theory classes. The problems will be proposed by the teacher from the proposed problems available in a collection of exercises available in Moodle. The latter will also allow autonomous student learning through the autonomous resolution of exercises out of the seminar sessions.

Tutories:

Individual sessions or small groups for the resolution of doubts related to the subject.

Homework:

Knowledge will be achieved through autonomous learning by students based on literature searches and the realization of a homework in group


The use of AI is permitted to improve the drafting and style of the reports and homeworks. However, generating original content with AI is strictly prohibited. If AI tools are used, their use must be explicitly declared, and it must be clearly identified within the report what they were used for.

Annotation: within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.

Assessment

Continuous assessment activities

Title Weight Hours ECTS Learning outcomes
Three partial exams 84% of final mark 9 0.36 CM21, KM19, KM20, SM19
Homework 16% of final mark 0 0 CM21, KM19, KM20

A) Continuous evaluation:

- Three partial exams that will count 84% of the final mark

- A group homework that will count 16% of the final mark

The scheduling of the assessment activities will be given on the first day of the subject and will be made public through the web of the faculty of biosciences.

B) Recovery test:

The group homework is not recoverable.

The student can submit to recovery test whenever it has been presented to a set of activities that represent a minimum of two thirds of the total grade of the subject. Of these, students who have an average qualification of all the activities of the subject superior to 3,5 on 10 will be able to present to the recovery final test.

Only students who fail the continuous evaluation can submit the recovery test.

The synthesis exam (recovery) includes the entire contents of the subject.


C) Procedure for the revision of the qualifications:

For each assessment activity, a place, date and time of revision will be indicated in which the student will be able to review the activity with the teacher. In this context, claims can be made about the activity note, which will be evaluated by the teachers responsible for the subject. If the student does not submit to this review, this activity will not be reviewed later.

D) Qualifications:

The MH qualification is a decision of the lecturers responsible for the subject. The regulations of the UAB indicate that MH can only be awarded to students who have obtained a final grade of 9.00 or more. It can be granted up to 5% of MH of the total students enrolled.

A student will be considered non-evaluable (NA) if it has not been presented in a set of activities whose weight equals to a minimum of two thirds of the total grade of the subject.


E) Irregularity in an assessment activity

Committing any irregularity in an assessment activity (academic fraud, plagiarism, or misuse of AI, unless such use is expressly authorized in the teaching guide) that could lead to a significant variation in the grade, will result in this activity being graded as 0. In the event that the teaching guide specifies that passing the subject requires a minimum grade in that particular assessment activity, or if multiple irregularities occur across different assessment activities within the same subject, the final grade for the subject will be 0. Aside from this, disciplinary proceedings may be initiated against any student who commits any of these irregularities.

Bibliography

-        Francesc Recasens.\"Processos de separació en biotecnologia industrial\". Iniciativa Digital Politècnica, Barcelona, 2015

-        Belter, P.A., Cussler, E.L., Wei-Shou Hu. “Bioseparations: Downstream Processigng for Biotechnology”. John Wiley and Sons, New York, 1988.

 -       Verrall, M.S., Hudson, M.J. Eds. “Separations for Biotechnology”.  Ellis Horwood Limited, UK, 1987.

 -       Gòdia, F., López, J. Eds. “Ingeniería Bioquímica”, 1998, Editorial Síntesis, Madrid.

 -       Blanch, H.W., Clark, D.S. “Biochemical Engineering”, 1996, Marcel Dekker, New York.

 -       Bailey, J.E., Ollis, D.F. “Biochemical Engineering Fundamentals”, 2ª Ed., 1986, McGraw Hill Book Company, New York.

 

E-Books (UAB):

Biochemical Engineering - A Textbook for EngineersChemists and Biologists

By: Shigeo Katoh; Fumitake Yoshida. 

WILEY-VCH Verlag GmbH & Co. KGaA John Wiley & Sons Incorporated. 

ISBN: 978-3-527-32536-8, 978-1-282-45667-9, 978-3-527-62764-6, 978-3-527-62765-3. 

http://web.b.ebscohost.com/pfi/detail/detail?vid=5&sid=52c4511f-c253-40f5-9394-357379fdd89e%40pdc-v-sessmgr03&bdata=JnNpdGU9cGZpLWxpdmU%3d#AN=edp657681&db=edspub

 

Procesos de separación de biotecnología industrial

By: Recasens, Francesc. 

Universitat Politècnica de Catalunya. 

ISBN: 978-84-9880-696-0.

http://web.a.ebscohost.com/pfi/results?vid=3&sid=2ec26e13-9d3f-498e-93a6-767b1a34d436%40sessionmgr4007&bquery=Procesos+de+separación+de+biotecnolog%C3%ADa+industrial&bdata=JmRiPWVkc3B1YiZ0eXBlPTQ0JnNlYXJjaE1vZGU9QW5kJnNpdGU9cGZpLWxpdmU%3d

 

Bioseparations Science and Engineering; Roger G. Harrison ... Et Al.

By: Roger G. Harrison; Demetri P. Petrides; Scott R. Rudge; Paul W. Todd. 

Oxford University Press. 

ISBN: 978-0-19-539181-7, 978-0-19-021373-2, 978-0-19-021374-9, 978-0-19-756276-5, 978-1-68015-865-6.

http://web.b.ebscohost.com/pfi/results?vid=1&sid=46411d38-83d3-44b0-9f40-f97fcfbb5184%40sessionmgr102&bquery=Bioseparations+Science+and+Engineering%3b+Roger+G.+Harrison+...+Et+Al.&bdata=JmRiPWVkc3B1YiZ0eXBlPTQ0JnNlYXJjaE1vZGU9QW5kJnNpdGU9cGZpLWxpdmU%3d

 

 

Software

MS Office. 

Course groups and languages

The information provided is provisional until November 30. After this date, you will be able to consult the language of each group through this link. To access the information, you will need to enter the course CODE

Type of teaching Group Language Semester Shift
(TE) Theory 43 Spanish first semester morning-mixed
(PAUL) Classroom practices 431 Catalan/Spanish first semester morning-mixed
(PAUL) Classroom practices 432 Catalan/Spanish first semester morning-mixed