The subject of cell cultures consists of theoretical master classes (10 hours) using audiovisual materials and practical classes in the laboratory (16 hours)

Theoretical Classes:

The theoretical master classes will be conducted using audiovisual material prepared by the professor. This material will be available to students on the Virtual Campus (CV) of the UAB before each session. It is recommended to consult the audiovisual material and the recommended books in the Bibliography section of this teaching guide to consolidate the content explained in class.

Practical Classes:

The practical classes for this subject are designed to help students learn to use laboratory instruments and enhance their theoretical knowledge. These practical classes are not associated with the Integrated Laboratory IV subject, as the cell culture subject is theoretical-practical.

There are 5 practical sessions totalling 16 hours, scheduled from Monday to Friday. Prior to starting the first session, a mandatory presentation session will be held for each group. The practical guide will be available in PDF format on the CV.

Each day, students will need to submit an exercise (via CV) or complete a questionnaire (in the classroom) at the beginning of each practical session related to the day’s experiments. These activities will be evaluated and contribute to the final grade for the subject. Late arrival to the session will result in the inability to complete the questionnaires.

Students will work in pairs. Each pair must submit a report showing and discussing the results obtained, both individually and as part of the practical group. The goal of this activity is to promote scientific reasoning at both the individual and team levels.

 Tutorials:

Tutorials will be personalized and held in the professor’soffice (Room C2/042) at a convenient time. They should be used to clarify concepts, reinforce acquired knowledge, and facilitate student study.

To pass the course, it is necessary to obtain a minimum overall score of 5.0 out of 10. The final grade will be calculated according to the following weighting:

Final grade = Theory exams (30% + 30%) + Laboratory practicals (10% + 30%)

Assessment activities

1) Theory exams (60%). They consist of two distinct parts:

a) Knowledge exam (30%). Test on the different topics taught in the theory classes. A minimum grade of 4.0 is required in order to be averaged with the rest of the activities.

b) Application of knowledge exam (30%). Resolution of a practical case study. No minimum grade is required to be averaged. This activity is not subject to retake.

2) Laboratory practicals (40%)

a) Practical report (10%). The grading of this activity is broken down as follows:

• 95% corresponds to the practical report.
• 5% corresponds to the pre-session quizzes for each session*.

No minimum grade is required to be averaged. This activity is not subject to retake.

b) Laboratory techniques exam (30%). Written test on the techniques and results covered during the practical sessions. No minimum grade is required to be averaged. This activity is not subject to retake.

Attendance and preparation conditions for the practical sessions

Attendance at the practical sessions is mandatory. Punctuality is essential, as experimental methodologies are explained during the first 30 minutes.

Before each session, it will be necessary to complete a pre-session quiz on the practical of the day. To prepare it properly, students must:

• Read the practical guide.
• Watch the corresponding videos before each session.

*Assessment of the quizzes. Each quiz will be graded with a maximum of 0.1 points, up to a maximum of 0.5 points in total, which will be integrated into the grade of the practical report activity. Late arrival penalties

• More than 10 minutes late: penalty of 0.1 points in the practical grade and impossibility to complete the quiz.
• More than 30 minutes late: penalty of 0.3 points in the practical grade.

Students will receive the grade “Not assessable” if they attend less than 80% of the practical sessions.

Retake

To be eligible for a retake, students must have been assessed in activities that represent at least two thirds of the final grade. Otherwise, they will receive the grade “Not assessable”.

A resit exam is foreseen for:

• Students with a grade lower than 4.0 in the knowledge exam.
• Students who, despite having a minimum grade of 4.0 in this exam, do not achieve a final grade of 5.0.

The following are not subject to resit:

• The application of knowledge exam.
• Laboratory practical activities, due to their nature.

Use of AI: In this course, the use of Artificial Intelligence (AI) technologies is not allowed at any stage. Any work that includes AI-generated content will be considered a breach of academic integrity and may result in a partial or total penalty in the grade for the activity, or more severe sanctions in cases of serious misconduct.

VERY IMORTANT: The commission of any irregularity in an assessment activity (academic fraud, plagiarism, or improper use of AI, unless such use is expressly authorized in the course guide) that may lead to a significant alteration of the grade will result in that activity being graded as 0. In cases where the course guide establishes that obtaining a minimum grade in this assessment activity is an essential requirement to pass the course, or when multiple irregularities occur in the assessment activities of the same course, the final grade for the course will be 0. In addition, disciplinary proceedings may be initiated against any student who engages in any of these irregularities.