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Analytical Nanobiosystems

Code: 106830
Credits: 6
2026/2027
Degree programme Type Course
Nanoscience and Nanotechnology OP 4

Contact lecturer

Name :
Manel del Valle Zafra
Email :
manel.delvalle@uab.cat

Teaching staff

Maria del Mar Puyol Bosch

Group languages

You can consult this information at the end of the document.

Prerequisites

To enroll in any fourth-year course, you must have passed a minimum of 120 ECTS credits and have completed all of the first year.

It is advisable to have passed the courses Analytical Chemistry and Supramolecular Chemistry and Molecular Recognition.

Objectives

To apply the concepts, principles, theories and fundamental facts related to nanoscience and nanotechnology in the systems for chemical analysis and diagnosis. Familiarize yourself with the new bio-inspired analytical systems. To illustrate this application of the convergence of technologies that combines nanomaterials and biotechnology.

Learning outcomes

  • CM22 (Identify innovations in nanobiotechnology and their economic and social impact on the field of health.) Identify innovations in nanobiotechnology and their economic and social impact on the field of health.
  • KM42 (Recognise the application of analytical nano-biosystems for large-scale analysis.) Recognise the application of analytical nano-biosystems for large-scale analysis.
  • SM32 (Use digital tools and documentary sources to obtain, analyse and present information from a critical perspective in the field of nano biotechnology, both orally and in writing.) Use digital tools and documentary sources to obtain, analyse and present information from a critical perspective in the field of nano biotechnology, both orally and in writing.
  • SM36 (Identify and design nanomaterials and nano-systems applied to diagnosis and treatment in the field of biomedicine.) Identify and design nanomaterials and nano-systems applied to diagnosis and treatment in the field of biomedicine.

Contents

The syllabus of the subject is subdivided into the following lessons:


1. Integration in analytical chemistry. Integration of the analytical process. Chemical sensors and biosensors. Immobilization. Miniaturization. Multiplexed (bio)sensors and micro / nanosystems.

2. The biological element: use of cells, enzymes - detection of substrates or inhibitors. Antibodies and other proteins for recognition, oligonucleotides and aptamers, molecularly imprinted polymers.

3. Nanomaterials and nanofabrication: nanoparticles, quantum dots, magnetic particles, fullerenes, nanotubes, nanoespheres, nanowires, graphene, dendrimers, nanoarrays, nanopores.

4. Surface phenomena in systems for analysis. Self-assembled monolayers. Langmuir-Blodgett films. Liposomes. Functionalization of surfaces. Printing and lithography at the nanometric scale.

5. Immobilization of biomolecules. General principles of immobilization. Non-covalent immobilization: adsorption, entrapment. Sol-gel matrices. Covalent immobilization via amino or thiol groups. Click-chemistry reactions. Affinity: avidin-biotin interaction, hexahistidine group and Ni (II). Strategies for oriented immobilization.

6. Analytical methodologies using nanomaterials. Labeling. Competitive, capture or signaling strategies. Reduction of non-specific interaction. Blocking. Stabilization. Amplification of signals.

7. Systems with electrochemical transduction. Potentiometry: Selective electrodes and FETs. Voltammetry. Systems with enrichment. Electrochemical impedance spectroscopy.

8. Systems with optical transduction. Principles of optical measurement with the use of nanocomponents. Continuous methods and with resolution of time. Fluorescence Methods: FRET, "up-converting" fluorophores. Evanescent wave: SPR and SERS.

9. Other principles of transduction. Systems with mass transduction. Quartz microbalance and surface acoustic wave sensors. Systems with magnetic transduction. Bioinspired Systems: Electronic noses and electronic tongues.

10. Biosensors based on Nanoporus. Nanometric porosity arrays. Intercalation of Ion channel proteins. Stochastic biosensors. Applications in sequencing.

11. Systems with micro and nanofluidics. Lateral flow devices. Lab on a chip and on a CD. Electrophoresis on chip. Chips in genomics and proteomics. Field-flow fractionation.

12. Nanobiosensors for clinical diagnosis. Theranostics. Glucose and metabolites, protein markers, cancer markers, DNA, viruses, bacteria, isolation and CTC detection. Application of analytical nanobiosystems for large scale analysis.

Learning activities and methodology

Title Hours ECTS Learning outcomes
Problem classes 12 0.48 CM22, KM42, SM32, SM36
Use of information for oral presentations 12 0.48 CM22, KM42, SM32, SM36
Theoretical lectures 32 1.28 CM22, KM42, SM36
Tutorials 6 0.24 CM22, KM42, SM32, SM36
Preparation of oral presentations 12 0.48 CM22, KM42, SM32, SM36
Troubleshooting 20 0.8 CM22, KM42, SM36
Laboratory 12 0.48 CM22, KM42, SM32, SM36
Personal study 40 1.6 CM22, KM42, SM36

Students will have to carry out various types of activities throughout this subject:


a) Directed activities: In the classroom, there will be lectures on the contents of the subject (materials deposited on the virtual campus) and problem classes.


In the problem classes, numerical exercises will be worked on the contents of the course, and students will also make presentations of scientific articles related to the subject.


For each of the lessons 7 to 12 (inclusive), the teacher will prepare a list of scientific articles. Each student will choose one of the articles, and these works will be presented and analyzed in an individual oral presentation of 10-15 minutes, with a question and answer session, so that each student makes several presentations throughout the course.


As laboratory sessions, Analytical Chemistry laboratories that conduct research related to Nanoscience and Nanotechnology are visited, where nanomaterials and nanocomponents are available in the first session, and nanobiosystems for analysis in the second.


b) Supervised activities: Tutorials will be held in order to monitor one of the evaluation activities that students must carry out, which consists of reading, understanding and presenting orally a scientific article related to the subject.


c) Autonomous activities: Students must independently study the contents of the subject, solve problems, and prepare several presentations on scientific articles related to the subject.


Note: 15 minutes of a class will be reserved, within the calendar established by the center/degree, for students to complete the evaluation surveys of the performance of the teaching staff and the evaluation of the subject.


Use of AI


For this subject, the use of Artificial Intelligence (AI) technologies is allowed exclusively in support tasks, such as bibliographic or information search, text correction or translations. In the case that Ai is used, the student will have to clearly identify which parts have been generated with this technology, specify the tools used and include a critical reflection on how these have influenced the process and the final result of the activity. The non-transparency of the use of AI in an evaluable activity will be considered lack of academic honesty and may lead to a partial or total penalty in the grade of the activity.Use of AI


Annotation: within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.

Assessment

Continuous assessment activities

Title Weight Hours ECTS Learning outcomes
Oral presentations 40% 2 0.08 CM22, KM42, SM32, SM36
Quizzes 60% 2 0.08 CM22, KM42, SM36
Laboratory multiplicative coefficient (between 0.90-1.10) 0 0 CM22, KM42, SM36

The assessment will consist of the following components:


1. Quizzes: At the end of each topic, a multiple-choice quiz will be administered using the virtual campus tools. The quizz will last 10 minutes. All scheduled quizzes will count towards the final grade, except for the one with the lowest score. (60% of the final grade).


2. Oral presentations: Each student will give several presentations throughout the course, lasting 10-15 minutes, summarizing representative scientific articles from the syllabus. The articles, corresponding to lessons 7, 8, 9, 10, 11, and 12, will be chosen by the students from a list provided by the instructor. (40% of the final grade).


3. The laboratory component will be reflected in a multiplicative coefficient (between 0.90 and 1.10) of the final grade.


Students who have opted for the single assessment option must take a final exam consisting of a multiple-choice test and a second test in the form of short-answer questions. This test will be administered on the same day that students taking the continuous assessment exam take their second midterm exam. Upon completion, students will submit a number of written assignments equivalent to those given by their classmates as oral presentations, but in the form of critical commentaries on their chosen scientific work. The student's grade will be the weighted average of the three previous activities, with the multiple-choice exam accounting for 33%, the short-answer exam for 33%, and the written assignments for the remaining 34%.


If the final grade is below 5, the student has another opportunity to pass the course through a resit exam, which will be held on a date set by the Degree Coordinator. In this exam, 66% of the grade corresponding to the theory and problem-solving sections can be recovered. The written assignments are not eligible for a resit.


Any irregularity committed during an assessment activity (academic fraud, plagiarism, or the improper use of AI—unless such use is expressly authorized in the course syllabus) that could lead to a significant change in the grade will result in a grade of 0 for that activity. If the course syllabus stipulates that achieving a minimum grade in that specific assessment activity is a mandatory requirement for passing the course, or if multiple irregularities occur across assessment activities for the same course, the final course grade will be 0. Furthermore, disciplinary proceedings may be initiated against any student who commits such irregularities.

Bibliography

Nanobiosensing. Principles, development and application

H. Ju, X. Zhang, J. Wang

Springer, Heidelberg, 2011

ISBN 978-1-4419-9621-3


Nanomaterials for biosensors

C. Kumar

VCH Verlag, Weinheim, 2007

ISBN 978-3-527-31388-4


Chemical Sensors

P. Gründler

Springer, Heidelberg, 2007

ISBN 978-3-540-45742-8


Chemical Sensors and Biosensors: Fundamentals and Applications

F.G. Bănică

Wiley, Chichester, 2012

ISBN 978-0-470-71067-8

Software

N/A

Course groups and languages

The information provided is provisional until November 30. After this date, you will be able to consult the language of each group through this link. To access the information, you will need to enter the course CODE

Type of teaching Group Language Semester Shift
(TE) Theory 1 Catalan second semester morning-mixed
(PAUL) Classroom practices 1 Catalan second semester morning-mixed
(PLAB) Practical laboratories 1 Catalan second semester afternoon