
Integrated Laboratory I
Code: 101947Credits: 3
| Degree programme | Type | Course |
|---|---|---|
| Genetics | OB | 1 |
Contact lecturer
- Name :
- María Pilar Garcia Guerreiro
- Email :
- mariapilar.garcia.guerreiro@uab.cat
Teaching staff
- Laura Tusell Padros
- Isidre Gibert Gonzalez
Group languages
You can consult this information at the end of the document.
Prerequisites
- Students must have passed or are currently taking the theoretical courses corresponding to the laboratory contents.
- Student must have passed the laboratory safety and biosecurity test, and be knowledgeable and accept the laboratories operating regulations at the Biosciences School. The test and the information needed to properly answer its questions can be found in the “Campus Virtual” area (http://cv.uab.cat).
- Students who do not wear a laboratory coat/glasses cannot enter the laboratory.
Objectives
The Integrated Laboratory I is the first laboratory course of a set of six that are distributed throughout six semesters of the first three years of the Genetics Degree.
These laboratory courses aim to provide a solid basis for experimental procedures, techniques and skills in genetics and related sciences.
The laboratory practices reinforce the theoretical concepts acquired in the theoretical classes, allowing to fully understanding the essential dialogue between theory and experimentation that comprise the genetics science.
The Integrated Laboratory I has as its training objectives the acquisition of experimental competencies in 3 specific modules:
- Cell Biology
- Histology
- Microbiology
Cell Biology
1. To apply microscopic and cell culture techniques to recognize and describe structures and processes at the level of cell.
Histology
1. To know how to apply basic histological techniques for microscopic diagnosis.
2. Identify at the microscope different levels of animal tissues and their cellular and extracellular components.
Microbiology
1. To apply the general techniques of microorganisms culture, observation, identification and conservation.
Learning outcomes
- CM19 (Design experimental strategies and data analysis procedures for solving research problems in genetics.) Design experimental strategies and data analysis procedures for solving research problems in genetics.
- CM20 (Evaluate the relevance, limitations, and biological significance of data obtained using experimental and computational techniques.) Evaluate the relevance, limitations, and biological significance of data obtained using experimental and computational techniques.
- KM14 (Describe the methodologies of sample preparation, cytogenetics and microscopy applied to the diagnosis and analysis of human genetic variation.) Describe the methodologies of sample preparation, cytogenetics and microscopy applied to the diagnosis and analysis of human genetic variation.
- SM16 (Apply safety, biosafety and waste management protocols in the handling and processing of biological samples.) Apply safety, biosafety and waste management protocols in the handling and processing of biological samples.
Contents
Cell Biology module
Practice 1: Cellular diversity under the conventional optical microscope: The plant cell.
Practice 2: Cellular diversity under the conventional optical microscope: The animal cell.
Practice 3: Transport through the membrane: osmosis and diffusion.
Practice 4: Introduction to the electronic microscope.
Practice 5: Mitotic cell division.
Practice 6: Meiotic cell division.
Practice 7: Cell fragmentation and separation of organelles
Practice 8: Cultures: Basic techniques of cell cultures (counting and viability).
Histology module
Practice 1: Initiation to the histological techniques for processing animal material. Microscopic identification of
epithelial tissues.
Practice 2: Microscopic identification of connective and adipose tissues.
Practice 3: Elaboration and staining of blood smears of sheep. Microscopic identification of elements of blood and
cartilage and bone tissues.
Practice 4: Microscopic identification of muscular and nervous tissues.
Microbiology module
Practice 1: Basic equipment
Practice 2: Sterilization techniques
Practice 3: Culture media
Practice 4: Counting microorganisms
Practice 5: Isolation and conservation of microorganisms
Practice 6: Observation of microorganisms: stains
Practice 7: Observation of microorganisms: motility
Practice 8: Identification of microorganisms
Practice 9: Ubiquity and microbial diversity
Practice 10: Bacterial growth curve
Practice 11: Antibiotic Susceptibility Testing: diffusion method
Learning activities and methodology
| Title | Hours | ECTS | Learning outcomes |
|---|---|---|---|
| Laboratory notebook | 4 | 0.16 | KM14 |
| Study | 19 | 0.76 | CM19, SM16 |
| Individual tutorials | 1 | 0.04 | CM19, CM20, SM16 |
| Laboratory (Cell Biol 16h, Hist 14h, Microbiology 15h) | 45 | 1.8 | CM20, KM14, SM16 |
The subject is taught in small groups of students (maximum 20 per session) in the laboratory. Students have a manual or practice guide for each Module. It is necessary to read the corresponding part of each session carefully before starting the practice to obtain the maximum advantage.
The activities follow an autonomous process based on guided observation and experimentation. Students will have to elaborate the results obtained and / or respond to the questions posed in the scripts or the memoirs.
Once the teacher has begun the explanation of the practice, the entrance of no more student in the classroom will be allowed. The late student may retrieve the practice another day, either with a group of their own Degree or another, as long as the group is not full. In case all the remaining groups are full or the practice is no longer taught, the student will not be able to recover the practice and will have a lack of assistance.
If a student, due to justified cause, has not been able to attend a practical session, he must contact the person in charge of the subject and present the corresponding document. In this case, a new day will be assigned to recover the practice. In case the practice is no longer taught and the student can not recover it, it will not be counted as a lack of assistance.
Note: Health problems are considered justified by reason (the corresponding medical justification must be presented) or serious personal problems.
Assessment
Continuous assessment activities
| Title | Weight | Hours | ECTS | Learning outcomes |
|---|---|---|---|---|
| Histology. Individual continuous assessment throughout the course | 33.3% | 1.25 | 0.05 | CM20, KM14, SM16 |
| Final test (individual assessment) | 100% | 2 | 0.08 | CM19, CM20, KM14, SM16 |
| Microbiology. Individual continuous assessment throughout the course | 33.3% | 1.33 | 0.053 | CM19, CM20, KM14, SM16 |
| Cell Biology. Individual continuous assessment throughout the course | 33.3% | 1.42 | 0.057 | CM20, KM14, SM16 |
Cell Biology Module
-At the end of each practice, students must complete a questionnaire and/or file to assess whether they have assimilated the specific objectives that have been worked on.
-Students who do not attend any of the sessions without a justified cause will have a zero on the questionnaire.
-An absence in the practical sessions of BC greater than 20%, whether justified or unjustified, will lead to a qualification of Not Assessable.
-The final grade for the Cell Biology module will be calculated from the average grade of the 8 questionnaires and/or practice sheets.
-Students who have obtained a final module grade of less than 5.0 (out of 10) may take a recovery exam on the scheduled date at the end of the semester. The recovery of module 1 (Cellular Biology) will consist of a written exam in which the concepts worked on in the practical sessions will be evaluated.
Histology module: The evaluation system is organized in the following sections:
1) Assessment of the contents at the end of each practice (50% of the mark). This test consists of a questionnaire and the recognition of microscopic structures. The note in this section is obtained from the average of the grades obtained in each practice. If you do not attend any of the sessions, without just cause, the corresponding note of the practice will be considered as zero.
2) Global microscopic diagnostic test (50% of the note).
In order to be able to gauge the notes obtained in each section, it will be essential that the student obtains a grade equal to or greater than 4 points (out of 10) in each of them. Students who have obtained a final grade of less than 5 (out of 10) will have to take a recovery test, which will consist of a microscopic diagnostic test and a questionnaire.
Microbiology module: There will be two types of evaluation:
1. Continuousassessment of group work. The practical ability of each group of students will be evaluated daily, taking into account the results obtained in each session.
2. Individual assessment of the contents: a written essay will be performed on the last day of practice, consisting of several test questions.
These tests will have a weight of 3 and 7 points, over 10, respectively.
General subject considerations
To approve the Integrated Laboratory, it is necessary to have approved each one of the three modules with a mark ≥ 5. The final grade will be the average of the notes of each module
To be eligible for the retake process, the student should have been previously evaluated in a set of activities equalling at least two thirds of the final score of the course or module. Thus, the student will be graded as \"No Avaluable\" if the weigh thin of all conducted evaluation activities is less than 67% of the final score.
Attendance to practical sessions is mandatory. Students missing more than 20% of programmed sessions will be graded as \"No Avaluable\".
Single assessment
Students who take the single assessment must do the laboratory practices in face-to-face sessions, as they are teaching activities with compulsory attendance. This unique assessment system will be applied to module 3 (Microbiology), and will consist of a unique synthesis test with test-type or developmental questions on the contents of the practical activities and microscopic diagnosis (if they can be done from of printed images). The grade obtained in this summary test will have the same weight in the final grade as those established for the continuous assessment.
The single assessment test will take place on a date agreed between the students, the subject coordinator and those responsible for Microbiology module.
Thesame criterion will be applied to approve the subject and the same recovery system as for the continuous assessment. The review of the final qualification follows the same procedure as for the continuous assessment.
Modules 1 (Cell Biology) and 2 (Histology) do not provide for the single assessment system.
Any irregularity committed in an assessment activity (academic fraud, plagiarism, or improper use of AI, unless such use is expressly authorized in the study guide) that may lead to a significant alteration of the grade will result in that assessment activity being graded with a 0. If the study guide establishes that obtaining a minimum grade in that assessment activity is an essential requirement for passing the course, or if multiple irregularities occur in the assessment activities of the same course, the final grade for the course will be 0. In addition, disciplinary proceedings may be initiated against any student who engages in any of these irregularities.
Bibliography
Cell Biology module
The practice guide can be downloaded from the Virtual Campus.
Histology module
The Dossier of activities can be downloaded from the Virtual Campus
Boya, J.: Atlas de Histología y Organografía microscópica (ed. Panamericana).
- Eroschenko, V.P.: Di Fiore’s atlas of Histology (ed. Lea and Febiger).
- Gartner, L.P. y Hiatt, J.L.: Atlas color de Histología (ed. Panamericana).
- Kühnel, W.: Atlas color de Citología e Histología (ed. Panamericana).
- Stanley, L.E. y Magney, J.E.: Coloratlas Histología (ed. Mosby).
- Young, B. y Heath, J.W.: Histología funcional (Wheater) (ed. Churchill Livingstone).
Microbiology Module
The practice guide can be downloaded from the Virtual Campus
Recommended Bibliography
- Michael T. Madigan. 2022. Brock. Biología de los microorganismos.16a Ed. ISBN: 9788490352793. Pearson Educación, SA. (https://bibcercador.uab.cat/permalink/34CSUC_UAB/avjcib/alma991010567908206709)
- Willey, J, LM Sherwood, CJ Woolverton. 2020. Microbiología de Prescott, Harley y Klein.11a ed. MacGraw-Hill. ISBN: 978-8448168278. (https://bibcercador.uab.cat/permalink/34CSUC_UAB/avjcib/alma991010721335606709)
Software
No specific software is needed.
Course groups and languages
The information provided is provisional until November 30. After this date, you will be able to consult the language of each group through this link. To access the information, you will need to enter the course CODE
| Type of teaching | Group | Language | Semester | Shift |
|---|---|---|---|---|
| (PLAB) Practical laboratories | 611 | Catalan/Spanish | first semester | morning-mixed |
| (PLABs) Suport a les pràctiques de laboratori | 611 | Catalan/Spanish | first semester | morning-mixed |
| (PLAB) Practical laboratories | 612 | Catalan/Spanish | first semester | morning-mixed |
| (PLABs) Suport a les pràctiques de laboratori | 612 | Catalan/Spanish | first semester | morning-mixed |
| (PLAB) Practical laboratories | 613 | Catalan/Spanish | first semester | morning-mixed |
| (PLABs) Suport a les pràctiques de laboratori | 613 | Catalan/Spanish | first semester | morning-mixed |