
Cellular Culture
Code: 100929Credits: 3
| Degree programme | Type | Course |
|---|---|---|
| Biotechnology | OB | 3 |
Contact lecturer
- Name :
- Laura Tusell Padros
- Email :
- laura.tusell@uab.cat
Group languages
You can consult this information at the end of the document.
Prerequisites
Erasmus students should consider that lessons are taught in Català.
Objectives
Cell Culture course is taught in the 2nd semester of the 3rd year of the Biotechnology degree in the Faculty of Biosciences. This is a subject with a certain degree of expertise that is intended to acquire a basic knowledge to work in a cell culture laboratory. It is therefore a subject with an important practical component.
Course objectives:
1) Know the basic equipment of a cell culture laboratory.
2) Know the most used types of cultures
3) Know the basic methodologies used in cell cultures.
Learning outcomes
- CM24 (Review the general safety standards of a biotechnology laboratory.) Review the general safety standards of a biotechnology laboratory.
- KM24 (Explain the theoretical foundation and appropriate techniques for the structural and functional characterization of proteins and nucleic acids.) Explain the theoretical foundation and appropriate techniques for the structural and functional characterization of proteins and nucleic acids.
- SM20 (Use the basic techniques of manipulation, separation, detection and analysis of proteins and nucleic acids.) Use the basic techniques of manipulation, separation, detection and analysis of proteins and nucleic acids.
- SM21 (Use prokaryotic and eukaryotic cell culture techniques and techniques for the manipulation of biological systems.) Use prokaryotic and eukaryotic cell culture techniques and techniques for the manipulation of biological systems.
- SM22 (Use analytical methodologies for the assay of biological activity of cellular components.) Use analytical methodologies for the assay of biological activity of cellular components.
Contents
THEORY CLASS PROGRAM: It basically consists of knowing the equipment, facilities, materials and techniques necessary for the manipulation and culture of animal cells and the use of biological material in sterile conditions.
Block I_INTRODUCTION
Unit 1. Brief history of cell cultures
Block II_ BASIC PRINCIPLES OF ANIMAL CELL CULTURES
Unit 2. 2D cultures: production and maintenance of cells
Unit 3. Physicochemical conditions of cell culture
Unit 4. Design of facilities and equipment
Block III_BASIC TECHNIQUES IN ANIMAL CELL CULTURES
Unit 5. Quantification, cytotoxicity test and cell death
Unit 6. Cryopreservation of cells
Unit 7. Contamination of crops
Unit 8. Characterization and authentication of cells
Block IV_ADVANCED TECHNIQUES IN ANIMAL CELL CULTURES
Unit 9. Selection and purification of cells
Unit 10. Cell cycle analysis and cell synchronization
Block V_BIOTECHNOLOGY IN ANIMAL CELLS
Unit 11. Cell lines in research and biotechnology production
Unit 12. Scale-up of cell cultures
Unit 13. Red biotechnology: application of cell cultures
PRACTICAL SESSIONS PROGRAM: Broadly speaking, the practices consist of the manipulation of animal eukaryotic cell cultures. The 5 laboratory sessions are designed so that the student complements his theoretical training with basic techniques and the tools of a cell culture laboratory. In the practical sessions you will work on:
- Subculture of animal cell lines in a biological safety cabinet.
- Establishment of a cell growth curve.
- Immunofluorescent detection of microtubules and observation in the epifluorescence microscope.
- Freezing and thawing of cells: recovery and survival rate.
- Induction and analysis of apoptosis.
- Alteration of the cell cycle by different drugs.
- Discussion of results.
Learning activities and methodology
| Title | Hours | ECTS | Learning outcomes |
|---|---|---|---|
| Lectures | 10 | 0.4 | CM24, KM24 |
| Practices | 16 | 0.64 | CM24, SM20, SM21, SM22 |
| Study and laboratory results | 46 | 1.84 | CM24, KM24 |
Given its theoretical-practical aspect, the Cell Cultures subject consists of both theoretical lectures -10 hours- and practical classes in the laboratory -16 hours- (see table of training activities).
- Theoretical classes are usually scheduled for the first three weeks of the second semester. The master classes will be carried out using audiovisual material prepared by the teaching staff, material that the students will have at their disposal in * pdf format in the Moodle of the subject before the start of the sessions. It is recommended that students consult the audiovisual material and the books recommended in the Bibliography section of the teaching guide or class slides to consolidate the contents explained in class.
- The practical classes of the subject are designed so that students learn to use laboratory instruments and complete their theoretical training. These practices are not associated with the Integrated Laboratory VI subject, since, as mentioned, the cell culture subject is theoretical-practical.
Practical classes will be scheduled in 4 turns of students and each one of the turns will consist of 5 sessions with a total of about 16 hours distributed from Monday to Friday. The list of students in each turn will be published in the Moodle of the subject. Group modifications are accepted but require exchange of students among groups.
The practice script will be available in *pdf format in Moodle. For the proper functioning and understanding of the practices, students must read the practice scriptcorresponding to each session and watch the recommended videos. Each day an exercise (via Moodle) or a questionnaire (in the classroom) must be handed at the beginning of each practical session on issues relatedto the day's experiments. These activities will be evaluated and will have a weight in the final grade of the subject. Delays in arriving at the practical sessions will lead to not being able to complete the questionnaires.
The students will work in groups of 2 people, and on a daily basis they will have to fill out a results sheet/form with the obtained data. The results obtained by the couple will be evaluated and will have a weight in the final grade of the subject. On the last day of practices, the results obtained in each of the practices will be assessed and discussed.
- The tutorials will be carried out in a personalized way in the teacher's office (door C2/050 and hours to be agreed). The tutorials should be used to clarify concepts, settle the knowledge acquired and facilitate the study by the students, among others.
Assessment
Continuous assessment activities
| Title | Weight | Hours | ECTS | Learning outcomes |
|---|---|---|---|---|
| Laboratory exam | 15% | 0.5 | 0.02 | CM24, SM20, SM21, SM22 |
| Laboratory exercices and questionnaires | 10% | 0.5 | 0.02 | SM20, SM21, SM22 |
| Theory test exam | 60% | 1 | 0.04 | CM24, KM24 |
| Case study | 5% | 0.5 | 0.02 | CM24, KM24, SM20, SM21, SM22 |
| Laboratory results | 10% | 0.5 | 0.02 | SM20, SM21, SM22 |
The evaluation will consist of activities corresponding to the theoretical part of the subject (65% of the overall grade) and to the practical part (35% of the overall grade). Thus, the evaluation will consider both the scientific knowledge acquired by each of the students individually and the results obtained and worked on in groups during the practices. Specifically, there will be:
Theory
It will consist of 2 differentiated parts that will evaluate the knowledge acquired on different concepts taught in theory classes and a practical case to be solved where different experiments or techniques will have to be determined to be applied to reach the expected results.
1) Theory exam (60% of the mark).
2) Practical case to solve (5% of the note).
Practices
3) Questionnaires and laboratory exercises (10% of the grade).
4) Results of practices (10% of the note).
5) Exam of practices and discussion of results (15% of the grade).
Students who do not hand in the questionnaires and/or results of the practices will have a zero for this part of the course.
It is noteworthy that the attendance at practical sessions is mandatory. The absence of one of the practical sessions implies a penalty of 25% of the mark in this section. Students will obtain the \"Non-Appraising\" qualification when their absence in the practical module exceeds 20% of the programmed sessions (2 or more sessions).
General considerations
To pass the course, a minimum grade of 4,0 out of 10 is required in the multiple choice exam of the theoretical assessment and that the weighted average of all 5 grades is equal to or greater than 5,0 points out of 10.
Recovery Activities
Students who initially fail the course may take the resit. However, to participate in the resit, students must have been previously assessed on a set of activities whose weight is equivalent to at least two-thirds of the total course grade (67%).
In summary, the resit will consist of a multiple-choice exam, which will assess the achievement of the learning objectives corresponding to the theory exam, and will have the same weight as the initial exam. All activities corresponding to the practical exercises (3, 4, and 5) and the case study (2) are excluded from the resit process.
The different scenarios for taking the resit are:
- that the theory exam grade was lower than 4.0 (out of 10).
- that the weighted average of the written tests (theory + case study) + practical exercises is lower than 5.0 points (out of 10). Finally, students who have passed the course and wish to improve their grade may do so, provided they notify the teaching staff one week in advance. It is important to note that in this context, students waive the grade obtained on the previous written theory exam.
For this course, the use of Artificial Intelligence (AI) technologies is permitted exclusively for support tasks, such as bibliographic or information searches, but is not allowed in graded assignments. Lack of transparency regarding the use of AI in graded activities will be considered a breach of academic integrity and may result in a partial or full penalty on the assignment grade, or more severe sanctions in serious cases.
Any irregularity committed during an assessment (academic fraud, plagiarism, or misuse of AI, unless expressly authorized in the course syllabus) that could lead to a significant change in the grade will result in a grade of 0 for that assessment. If the course syllabus stipulates that passing the course requires committing several irregularities in the assessments of the same course, the final grade for that course will be 0. Furthermore, disciplinary proceedings may be initiated against any student who commits any of these irregularities.
NB: This text has not been proofreading by a native English, so in case of any doubt or incongruity, the information provided in the Catalan/Spanish version will prevail.
Bibliography
-R.I. Freshney. Culture of Animal Cells: A manual of basic technique and specialized applications. 7th ed. Wiley-Blackwell. John Wiley & Sons, Inc. 2016. Free access to 6th ed (2010) for UAB students: https://onlinelibrary.wiley.com/doi/book/10.1002/9780470649367
-Specific bibliography is referenced in the class slides.
Software
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Course groups and languages
The information provided is provisional until November 30. After this date, you will be able to consult the language of each group through this link. To access the information, you will need to enter the course CODE
| Type of teaching | Group | Language | Semester | Shift |
|---|---|---|---|---|
| (TE) Theory | 43 | Catalan | second semester | morning-mixed |
| (PLAB) Practical laboratories | 431 | Catalan/Spanish | second semester | morning-mixed |
| (PLABs) Suport a les pràctiques de laboratori | 431 | Catalan/Spanish | second semester | morning-mixed |
| (PLAB) Practical laboratories | 432 | Catalan/Spanish | second semester | morning-mixed |
| (PLABs) Suport a les pràctiques de laboratori | 432 | Catalan/Spanish | second semester | morning-mixed |
| (PLAB) Practical laboratories | 433 | Catalan/Spanish | second semester | morning-mixed |
| (PLABs) Suport a les pràctiques de laboratori | 433 | Catalan/Spanish | second semester | morning-mixed |
| (PLAB) Practical laboratories | 434 | Catalan/Spanish | second semester | morning-mixed |
| (PLABs) Suport a les pràctiques de laboratori | 434 | Catalan/Spanish | second semester | morning-mixed |